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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/32610

    標題: Antimicrobial Activity of Lactobacillus Species Against Carbapenem-Resistant Enterobacteriaceae
    作者: Chen, Chi-Chung
    Lai, Chih-Cheng
    Hui-Ling Huang(黃惠玲)
    Wen-Yu Huang(黃雯玉)
    Toh, Han-Siong
    Weng, Tzu-Chieh
    Chuang, Yin-Ching
    Lu, Ying-Chen
    Tang, Hung-Jen
    貢獻者: Chi Mei Med Ctr, Dept Med Res
    Natl Chiayi Univ, Dept Food Sci
    Chi Mei Hosp, Dept Intens Care Med
    Chia Nan Univ Pharm & Sci, Dept Hlth & Nutr
    Chi Mei Med Ctr, Dept Intens Care Med
    Chi Mei Med Ctr, Dept Med
    關鍵字: Lactobacillus
    in vitro - antibacterial
    日期: 2019-04
    上傳時間: 2020-07-29 13:52:00 (UTC+8)
    摘要: Objective: This study aims to identify suitable lactobacilli that have anti-carbapenemresistant Enterobacteriaceae (CRE) activity with in vitro tolerance to pepsin and bile salts. Methods: Fifty-seven Lactobacillus spp. strains encompassing nine species were collected for investigation. Their viabilities in the presence of pepsin and bile salts were tested using tolerance tests. Their anti-CRE effects were assessed by agar well diffusion and broth microdilution assay, as well as time-kill test. Results: Of the 57 Lactobacillus isolates collected, 31 had a less than 2-log reduction in their viability in both pepsin and bile salt tolerance tests. Of these 31 isolates, 5 (LUC0180, LUC0219, LYC0289, LYC0413, and LYC1031) displayed the greatest anti-CRE activity with a CRE zone of inhibition greater than 15 mm in agar well diffusion assays. The minimal inhibitory percentages of supernatants from these five strains against CREs ranged from 10 to 30%. With the exception of LUC0180, which had a minimal bactericidal percentage >= 40%, the bactericidal percentage of all the strains ranged from 20 to 40%. The inhibitory effect of the cell-free culture supernatants from these Lactobacillus strains did not change after heating but was abolished as the pH changed to 7.0. After a 24-h incubation, five of the Lactobacillus strains at a concentration of 10(8) CFU/ml totally inhibited the growth of carbapenem-resistant Escherichia coil (CRE316) and Kiebsiella pneumoniae (CRE632). After a 48-h incubation, the growth of CRE316 was completely inhibited under each concentration of lactobacilli based on time-kill test. Furthermore, when the concentration of lactobacilli was at 10(8) CFU/ml, the decline in pH was faster than at other concentrations. Conclusion: Some Lactobacillus strains exhibit anti-CRE activity, which suggests potential applications for controlling or preventing CRE colonization or infection.
    關聯: Frontiers in Microbiology, v.10, Article789
    Appears in Collections:[保健營養系(所) ] 期刊論文

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