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| 標題: | A New Triterpenoid Glucoside from a Novel Acidic Glycosylation of Ganoderic Acid A via Recombinant Glycosyltransferase of Bacillus subtilis |
| 作者: | Chang, Te-Sheng
Chien-Min Chiang(江建民)
Kao, Yu-Han
Wu, Jiumn-Yih
Wu, Yu-Wei
Wang, Tzi-Yuan |
| 貢獻者: | Natl Univ Tainan, Dept Biol Sci & Technol
Chia Nan Univ Pharm & Sci, Dept Biotechnol
Natl Quemoy Univ, Dept Food Sci, Jinning 892, Kinmen County, Taiwan
Taipei Med Univ, Coll Med Sci & Technol, Grad Inst Biomed Informat
Taipei Med Univ Hosp, Clin Big Data Res Ctr
Acad Sinica, Biodivers Res Ctr |
| 關鍵字: | ganoderic acid A
glucosyltransferase
acidic
Bacillus subtilis
triterpenoid |
| 日期: | 2019-10 |
| 上傳時間: | 2020-07-29 13:51:03 (UTC+8) |
| 出版者: | MDPI |
| 摘要: | Ganoderic acid A (GAA) is a bioactive triterpenoid isolated from the medicinal fungus Ganoderma lucidum. Our previous study showed that the Bacillus subtilis ATCC (American type culture collection) 6633 strain could biotransform GAA into compound (1), GAA-15-O-beta -glucoside, and compound (2). Even though we identified two glycosyltransferases (GT) to catalyze the synthesis of GAA-15-O-beta -glucoside, the chemical structure of compound (2) and its corresponding enzyme remain elusive. In the present study, we identified BsGT110, a GT from the same B. subtilis strain, for the biotransformation of GAA into compound (2) through acidic glycosylation. BsGT110 showed an optimal glycosylation activity toward GAA at pH 6 but lost most of its activity at pH 8. Through a scaled-up production, compound (2) was successfully isolated using preparative high-performance liquid chromatography and identified to be a new triterpenoid glucoside (GAA-26-O-beta -glucoside) by mass and nuclear magnetic resonance spectroscopy. The results of kinetic experiments showed that the turnover number (k(cat)) of BsGT110 toward GAA at pH 6 (k(cat) = 11.2 min(-1)) was 3-fold higher than that at pH 7 (k(cat) = 3.8 min(-1)), indicating that the glycosylation activity of BsGT110 toward GAA was more active at acidic pH 6. In short, we determined that BsGT110 is a unique GT that plays a role in the glycosylation of triterpenoid at the C-26 position under acidic conditions, but loses most of this activity under alkaline ones, suggesting that acidic solutions may enhance the catalytic activity of this and similar types of GTs toward triterpenoids. |
| 關聯: | Molecules, v.24, n.19, 3457 |
| 顯示於類別: | [生物科技系(所)] 期刊論文
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