Exercise Preconditioning Attenuates Neurological Injury by Preserving Old and Newly Formed HSP72-Containing Neurons in Focal Brain Ischemia Rats

doi:10.7150/ijms.32962

Chia Nan University of Pharmacy & Science Institutional Repository > 環境永續暨休閒學院 > 休閒保健管理系(所) > 期刊論文 > Item 310902800/32579

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/32579

標題:	Exercise Preconditioning Attenuates Neurological Injury by Preserving Old and Newly Formed HSP72-Containing Neurons in Focal Brain Ischemia Rats
作者:	Wang, Yu-Lin Lin, Cheng-Hsien Chen, Chi-Chun Chang, Ching-Ping Lin, Kao-Chang Su, Fong-Chin Willy Chou(周偉倪)
貢獻者:	Natl Cheng Kung Univ, Dept Biomed Engn Southern Taiwan Univ Sci & Technol, Ctr Gen Educ Chi Mei Med Ctr, Dept Phys Med & Rehabil Mackay Med Coll, Dept Med Natl Chin Yi Univ Technol, Dept Elect Engn Chi Mei Med Ctr, Dept Med Res Chi Mei Med Ctr, Dept Neurol Natl Cheng Kung Univ, Med Device Innovat Ctr Chia Nan Univ Pharm & Sci, Dept Recreat & Healthcare Management
關鍵字:	Exercise heat shock protein 72 neuroprotection ischemic brain injury
日期:	2019
上傳時間:	2020-07-29 13:50:40 (UTC+8)
出版者:	IVYSPRING INT PUBL
摘要:	Background: Exercise preconditioning (EP+) is a useful and important procedure for the prevention of stroke. We aimed to ascertain whether EP+ protects against ischemic brain injury by preserving heat shock protein (HSP) 72-containing neurons in ischemic brain tissues. Methods: Adult male Sprague-Dawley rats (n=240) were used to assess the contribution of HSP72-containing neurons to the neuroprotective effects of EP+ on ischemic brain injury caused by transient middle cerebral artery occlusion. Results: Significant (P<0.05) increases in the percentages of both old HSP72-containing neurons (NeuN+HSP72 double positive cells) (18 similar to 20% vs. 40 similar to 50%) and newly formed HSP72-containing neurons (BrdU+NeuN+HSP72 triple positive cells); (2 similar to 3% vs. 16 similar to 20%) after 3 weeks of exercise coincided with significant (P<0.05) reductions in brain ischemia volume (250 mm(3) vs. 100 mm(3)), brain edema (78% vs. 74% brain water content), blood-brain barrier disruption (1.5 mu g/g vs. 0.7 mu g/g tissue Evans Blue dye extravasation) and neurological motor deficits (neurological severity scores of 12 vs. 6 and maximal angles of 60 degrees vs. 20 degrees) in brain ischemia rats. Reductions in the percentages of both old (from 40 similar to 50% to 10 similar to 12%) and newly formed (from 18 similar to 20% to 5 similar to 7%) HSP72-containing neurons by gene silencing with an intracerebral injection of pSUPER small interfering RNA showed a significant (P<0.05) reversal in the neuroprotective outcomes. Our data provide an inverse correlation between the EP+-mediated increases in both old and newly formed HSP72-containing neurons and the extent of cerebral ischemic injury. Conclusions: The percentages of both old and newly formed HSP72-containing neurons are inversely correlated with the outcomes of ischemic brain injury. Additionally, preischemic treadmill exercise improves the outcomes of ischemic brain injury by preserving both the old and newly formed HSP72-containing neurons in rats.
關聯:	International Journal of Medical Sciences, v.16, n.5, pp.675-685
显示于类别:	[休閒保健管理系(所)] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
10.7150-ijms.32962.pdf		2133Kb	Adobe PDF	334	检视/开启
index.html		0Kb	HTML	1640	检视/开启

在CNU IR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....