Bavachin attenuates LPS-induced inflammatory response and inhibits the activation of NLRP3 inflammasome in macrophages

doi:10.1016/j.phymed.2018.12.008

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Title:	Bavachin attenuates LPS-induced inflammatory response and inhibits the activation of NLRP3 inflammasome in macrophages
Authors:	Hung, Yung-Li Wang, Shu-Chi Suzuki, Katsuhiko Fang, Shih-Hua Chen, Chi-Shuo Cheng, Wei-Chung Chia-Cheng Su(蘇家震) Yeh, Hsin-Chih Tu, Hung-Pin Liu, Po-Len Huang, Ming-Yii Li, Chia-Yang
Contributors:	Waseda Univ, Grad Sch Sport Sci, Tokorozawa Kaohsiung Med Univ, Dept Med Lab Sci & Biotechnol Waseda Univ, Fac Sport Sci, Tokorozawa Natl Taiwan Univ Sport, Inst Athlet Natl Tsing Hua Univ, Dept Biomed Engn & Environm Sci China Med Univ, Grad Inst Biomed Sci Kaohsiung Med Univ, Coll Med, Grad Inst Med Chi Mei Med Ctr, Dept Surg, Div Urol Chia Nan Univ Pharm & Sci, Dept Senior Citizen Serv Management Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Urol Kaohsiung Med Univ, Coll Med, Sch Med, Dept Urol Kaohsiung Municipal Tatung Hosp, Dept Urol Kaohsiung Med Univ, Coll Med, Sch Med, Dept Publ Hlth & Environm Med Kaohsiung Med Univ, Coll Med, Dept Resp Therapy Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Canc Ctr, Dept Radiat Oncol Kaohsiung Med Univ Hosp, Dept Med Res Kaohsiung Med Univ, Ctr Infect Dis & Canc Res
Keywords:	Bavachin Macrophage Inflammation NLRP3 inflammasome mPGES-1
Date:	2019-06
Issue Date:	2020-07-29 13:48:26 (UTC+8)
Publisher:	ELSEVIER GMBH
Abstract:	Background: Bavachin is a natural product isolated from Psoralea corylifolia L. that has been applied as a traditional medicine in Asian countries. However, the anti-inflammatory effects of bavachin on LPS-induced inflammation and NLRP3 inflammasome activation by macrophages remain unclear. Purpose: We investigated the anti-inflammatory effects of bavachin on LPS-activated murine macrophage cell line J774A.1 cells and murine peritoneal macrophages. Methods: J774A.1 cells and murine peritoneal macrophages were pre-treated with bavachin following LPS treatment. The concentrations of NO, PGE(2), IL-6 and IL-12p40 in cell culture supernatant were analyzed. The expressions of iNOS, COX-2, mPGES-1 and MAPKs were analyzed using Western blotting, while NF-kappa B activity was detected using promoter reporter assay. To examine the activation of NLRP3 inflammasome, J774A.1 cells were incubated with LPS, and then treated with bavachin following treatment with ATP. The concentration of IL-1 beta in the cell culture supernatant was measured. The expressions of NLRP3, ASC, caspase-1 and IL-1 beta were analyzed using Western blotting. The formation of inflammasome complex was observed by immunofluorescence microscopy. Results: Bavachin suppressed LPS-induced NO and PGE(2) production, and decreased iNOS and mPGES-1 expression. Bavachin also reduced LPS-induced IL-6 and IL-12p40 production and decreased the activation of MAPKs and NF-kappa B. Additionally, bavachin suppressed NLRP3 inflammasome-derived IL-1 beta secretion, decreased caspase-1 activation, repressed mature IL-1 beta expression, and inhibited inflammasome complex formation. Furthermore, bavachin also suppressed the production of NO, IL-6 and IL-12p40 by LPS-stimulated murine peritoneal macrophages. Conclusion: Our experimental results indicated anti-inflammatory effects of bavachin exhibit attenuation of LPS-induced inflammation and inhibit activation of NLRP3 inflammasome in macrophages. These results suggest that bavachin might have potential in treating inflammatory and autoimmune diseases.
Relation:	Phytomedicine, v.59, 152785
Appears in Collections:	[Dept. of Senior Service and Health Management] Periodical Articles

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