Protein Expression Analysis in Reversible Photobleached Cells of Scenedesmus vacuolatus after High Temperature Stress

doi:10.3390/ijms20123082

CNU IR > Pharmacy and Science > Dept. of Cosmetic Science and institute of cosmetic science > Periodical Articles > Item 310902800/32517

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/32517

Title:	Protein Expression Analysis in Reversible Photobleached Cells of Scenedesmus vacuolatus after High Temperature Stress
Authors:	Ko, Tzu-Hsing Kuen-Lin Leu(呂昆霖) Hsu, Ban-Dar Lee, Tzan-Chain
Contributors:	Fujian Agr & Forestry Univ, Anxi Coll Tea Sci Chia Nan Univ Pharm & Sci, Dept Cosmet Sci Natl Tsing Hua Univ, Dept Life Sci
Keywords:	ATP synthase chlorophyll heat shock protein (HSP) heat stress photobleaching protein identification
Date:	2019-06
Issue Date:	2020-07-29 13:48:12 (UTC+8)
Publisher:	MDPI
Abstract:	We have analyzed protein expression in the bleached small vegetative cells of synchronous Scenedesmus vacuolatus to investigate how unicellular algae lived through stress. These cells were subjected to heat treatment (46.5 degrees C for 1h in dark condition) and then cultured under continuous illumination for 24 h. Flow cytometry analysis of the chlorophyll autofluorescence intensity of S. vacuolatus cells indicated that heat-treated cells were completely bleached within 24 h of light cultivation. Transmission electron microscopy (TEM) images showed that bleached cells maintained thylakoid membrane structure, but with lower contrast. The bleached cells regained green color after 72 h, along with a recovery in contrast, which indicated a return of photosynthetic ability. Two-dimensional gel electrophoresis (2DE) showed that the protein expression patterns were very difference between control and bleached cells. ATP synthase subunits and glutamine synthetase were down-regulated among the many differences, while some of phototransduction, stress response proteins were up-regulated in bleached cells, elucidating bleached cells can undergo changes in their biochemical activity, and activate some stress response proteins to survive the heat stress and then revive. In addition, small heat shock proteins (HSPs), but not HSP40 and HSP70 family proteins, protected the bleaching cells.
Relation:	International Journal of Molecular Sciences, v.20, n.12, 3082
Appears in Collections:	[Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles

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