Loading...
|
Please use this identifier to cite or link to this item:
https://ir.cnu.edu.tw/handle/310902800/32490
|
Title: | Potential Industrial Production of a Well-Soluble, Alkaline-Stable, and Anti-Inflammatory Isoflavone Glucoside from 8-Hydroxydaidzein Glucosylated by Recombinant Amylosucrase of Deinococcus geothermalis |
Authors: | Chang, Te-Sheng Wang, Tzi-Yuan Yang, Szu-Yi Kao, Yu-Han Wu, Jiumn-Yih Chien-Min Chiang(江建民) |
Contributors: | Natl Univ Tainan, Dept Biol Sci & Technol Acad Sinica, Biodivers Res Ctr Natl Quemoy Univ, Dept Food Sci Chia Nan Univ Pharm & Sci, Dept Biotechnol |
Keywords: | 8-hydroxydaidzein stable soluble anti-inflammation amylosucrase Deinococcus geothermalis |
Date: | 2019-06 |
Issue Date: | 2020-07-29 13:47:07 (UTC+8) |
Publisher: | MDPI |
Abstract: | 8-Hydroxydaidzein (8-OHDe), an ortho-hydroxylation derivative of soy isoflavone daidzein isolated from some fermented soybean foods, has been demonstrated to possess potent anti-inflammatory activity. However, the isoflavone aglycone is poorly soluble and unstable in alkaline solutions. To improve the aqueous solubility and stability of the functional isoflavone, 8-OHDe was glucosylated with recombinant amylosucrase of Deinococcus geothermalis (DgAS) with industrial sucrose, instead of expensive uridine diphosphate-glucose (UDP-glucose). One major product was produced from the biotransformation, and identified as 8-OHDe-7-alpha-glucoside, based on mass and nuclear magnetic resonance spectral analyses. The aqueous solubility and stability of the isoflavone glucoside were determined, and the results showed that the isoflavone glucoside was almost 4-fold more soluble and more than six-fold higher alkaline-stable than 8-OHDe. In addition, the anti-inflammatory activity of 8-OHDe-7-alpha-glucoside was also determined by the inhibition of lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells. The results showed that 8-OHDe-7-alpha-glucoside exhibited significant and dose-dependent inhibition on the production of nitric oxide, with an IC50 value of 173.2 mu M, which remained 20% of the anti-inflammatory activity of 8-OHDe. In conclusion, the well-soluble and alkaline-stable 8-OHDe-7-alpha-glucoside produced by recombinant DgAS with a cheap substrate, sucrose, as a sugar donor retains moderate anti-inflammatory activity, and could be used in industrial applications in the future. |
Relation: | Molecules, v.24, n.12, 2236 |
Appears in Collections: | [Dept. of Biotechnology (including master's program)] Periodical Articles
|
Files in This Item:
File |
Description |
Size | Format | |
10.3390-molecules24122236.pdf | | 2094Kb | Adobe PDF | 447 | View/Open | index.html | | 0Kb | HTML | 2696 | View/Open |
|
All items in CNU IR are protected by copyright, with all rights reserved.
|