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| 標題: | Potential Industrial Production of a Well-Soluble, Alkaline-Stable, and Anti-Inflammatory Isoflavone Glucoside from 8-Hydroxydaidzein Glucosylated by Recombinant Amylosucrase of Deinococcus geothermalis |
| 作者: | Chang, Te-Sheng
Wang, Tzi-Yuan
Yang, Szu-Yi
Kao, Yu-Han
Wu, Jiumn-Yih
Chien-Min Chiang(江建民) |
| 貢獻者: | Natl Univ Tainan, Dept Biol Sci & Technol
Acad Sinica, Biodivers Res Ctr
Natl Quemoy Univ, Dept Food Sci
Chia Nan Univ Pharm & Sci, Dept Biotechnol |
| 關鍵字: | 8-hydroxydaidzein
stable
soluble
anti-inflammation
amylosucrase
Deinococcus geothermalis |
| 日期: | 2019-06 |
| 上傳時間: | 2020-07-29 13:47:07 (UTC+8) |
| 出版者: | MDPI |
| 摘要: | 8-Hydroxydaidzein (8-OHDe), an ortho-hydroxylation derivative of soy isoflavone daidzein isolated from some fermented soybean foods, has been demonstrated to possess potent anti-inflammatory activity. However, the isoflavone aglycone is poorly soluble and unstable in alkaline solutions. To improve the aqueous solubility and stability of the functional isoflavone, 8-OHDe was glucosylated with recombinant amylosucrase of Deinococcus geothermalis (DgAS) with industrial sucrose, instead of expensive uridine diphosphate-glucose (UDP-glucose). One major product was produced from the biotransformation, and identified as 8-OHDe-7-alpha-glucoside, based on mass and nuclear magnetic resonance spectral analyses. The aqueous solubility and stability of the isoflavone glucoside were determined, and the results showed that the isoflavone glucoside was almost 4-fold more soluble and more than six-fold higher alkaline-stable than 8-OHDe. In addition, the anti-inflammatory activity of 8-OHDe-7-alpha-glucoside was also determined by the inhibition of lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells. The results showed that 8-OHDe-7-alpha-glucoside exhibited significant and dose-dependent inhibition on the production of nitric oxide, with an IC50 value of 173.2 mu M, which remained 20% of the anti-inflammatory activity of 8-OHDe. In conclusion, the well-soluble and alkaline-stable 8-OHDe-7-alpha-glucoside produced by recombinant DgAS with a cheap substrate, sucrose, as a sugar donor retains moderate anti-inflammatory activity, and could be used in industrial applications in the future. |
| 關聯: | Molecules, v.24, n.12, 2236 |
| 顯示於類別: | [生物科技系(所)] 期刊論文
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