Loss of cyclin-dependent kinase-like 2 predicts poor prognosis in gastric cancer, and its overexpression suppresses cells growth and invasion

doi:10.1002/cam4.1577

CNU IR > Pharmacy and Science > Dept. of Health and Nutrition (including master's program) > Periodical Articles > Item 310902800/32308

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Title:	Loss of cyclin-dependent kinase-like 2 predicts poor prognosis in gastric cancer, and its overexpression suppresses cells growth and invasion
Authors:	Fang, Chia-Lang Uen, Yih-Huei Chen, Han-Kun Hseu, You-Cheng Lin, Chih-Chan Hung, Shih-Ting Sun, Ding-Ping Lin, Kai-Yuan
Contributors:	Taipei Med Univ, Coll Med, Sch Med, Dept Pathol Taipei Med Univ, Wan Fang Hosp, Dept Pathol Asia Univ Hosp, Dept Surg Chi Mei Med Ctr, Dept Surg China Med Univ, Dept Cosmeceut Asia Univ, Dept Hlth & Nutr Biotechnol Chi Mei Med Ctr, Dept Med Res Chia Nan Univ Pharm & Sci, Dept Nutr Chia Nan Univ Pharm & Sci, Dept Biotechnol
Keywords:	CDKL2 gastric cancer immunohistochemistry prognosis
Date:	2018-07
Issue Date:	2019-11-15 15:49:00 (UTC+8)
Publisher:	WILEY
Abstract:	Cyclin-dependent kinase-like 2 (CDKL2), a new member of the cyclin-dependent kinase family, may be involved in gastric cancer (GC) progression. Thus, we conducted this study to explore the clinical effect of CDKL2 in GC. Immunohistochemistry was used to measure CDKL2 levels in gastric tissues. The association of a high CDKL2 level with clinical and pathological characteristics, and the correlation between the CDKL2 level and disease-free and overall survival were analyzed. Transfection was employed to overexpress CDKL2 in GC cells and to investigate the effect of CDKL2 overexpression on cell proliferation and invasion. Loss of CDKL2 was positively correlated with several clinical and pathological characteristics, and patients with a low CDKL2 level had significantly poorer disease-free and overall survival than those with a high level (P=.005 and .001, respectively). Univariate analysis using the Cox proportional hazards model indicated that a low CDKL2 level was a prognosticator for inferior disease-free survival (P=.007). Based on immmunoblotting data, AGS and HGC-27 GC cells were chosen for CDKL2 overexpression. Cellular studies revealed that CDKL2 overexpression impaired cell proliferation and invasion. Loss of CDKL2 may serve as a biomarker for predicting GC patient outcomes and a potential therapeutic target for GC treatment.
???metadata.dc.relation.uri???:	http://dx.doi.org/10.1002/cam4.1577
Relation:	Indian Journal of Pharmaceutical Sciences, v.7, n.7, pp.2993-3002
Appears in Collections:	[Dept. of Health and Nutrition (including master's program)] Periodical Articles [Dept. of Biotechnology (including master's program)] Periodical Articles

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