Regulatory Efficacy of Brown Seaweed Lessonia nigrescens Extract on the Gene Expression Profile and Intestinal Microflora in Type 2 Diabetic Mice

doi:10.1002/mnfr.201700730

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Title:	Regulatory Efficacy of Brown Seaweed Lessonia nigrescens Extract on the Gene Expression Profile and Intestinal Microflora in Type 2 Diabetic Mice
Authors:	Zhao, Chao Yang, Chengfeng Chen, Mingjun Lv, Xucong Liu, Bin Yi, Lunzhao Cornara, Laura Wei, Ming-Chi Yang, Yu-Chiao Tundis, Rosa Xiao, Jianbo
Contributors:	Fujian Agr & Forestry Univ, Coll Food Sci Univ Calif Davis, Dept Chem Quanzhou Normal Univ, Fujian Prov Key Lab Dev Bioact Mat Marine Algae Kunming Univ Sci & Technol, Yunnan Food Safety Res Inst Univ Genoa, Dipartimento Sci Terra Ambiente & Vita, Genoa Chia Nan Univ Pharm & Sci, Dept Appl Geoinformat Kaohsiung Med Univ, Dept & Grad Inst Pharmacol Univ Calabria, Dept Pharm Hlth & Nutr Sci, Arcavacata Di Rende Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Control Chinese Med, Ave Univ
Keywords:	anti-diabetic activity high-throughput 16S rRNA gene sequencing intestinal microflora Lessonia nigrescens signaling pathway
Date:	2018-02
Issue Date:	2019-11-15 15:48:58 (UTC+8)
Publisher:	WILEY
Abstract:	Scope: In this study, the antidiabetic activity of Lessonia nigrescens ethanolic extract (LNE) is investigated in streptozotocin (SZT)-induced type 2 diabetic mice fed with a high-sucrose/high-fat diet. Methods and results: Ultra high performance liquid chromatography coupled with photo-DAD and electospray ionization-mass spectrometry (ESI-MS) is employed to analyze the major compounds in LNE. The components of the intestinal microflora in type 2 diabetic mice are analyzed by high-throughput next-generation 16S rRNA gene sequencing. Fasting blood glucose levels in diabetic mice are significantly decreased after LNE administration. The histology reveals that LNE could protect the cellular architecture of liver and kidney. LNE treatment significantly increases Bacteroidetes and decreases Firmicutes populations in intestinal microflora. Specifically, It could selectively enrich the amounts of beneficial bacteria, Barnesiella, as well as reduce the abundances of Clostridium and Alistipes. The increased gene and protein expression levels of phosphatidylinositol 3-kinase (PI3K) in the liver are observed in LNE treatment groups, while the expressions of c-Jun N-terminal kinase (JNK) are significantly downregulated. Conclusion: The above findings suggest that LNE could be considered as a functional food for reducing blood glucose and regulating intestinal microflora.
???metadata.dc.relation.uri???:	http://dx.doi.org/10.1002/mnfr.201700730
Relation:	Cancer Medicine, v.62, n.4, 1700730
Appears in Collections:	[Dept. of Applied Geoinformatics] Periodical Articles

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