Ceramide synthase 6 predicts the prognosis of human gastric cancer: It functions as an oncoprotein by dysregulating the SOCS2/JAK2/STAT3 pathway

doi:10.1002/mc.22888

CNU IR > Pharmacy and Science > Dept. of Cosmetic Science and institute of cosmetic science > Periodical Articles > Item 310902800/32306

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Title:	Ceramide synthase 6 predicts the prognosis of human gastric cancer: It functions as an oncoprotein by dysregulating the SOCS2/JAK2/STAT3 pathway
Authors:	Uen, Yih-Huei Fang, Chia-Lang Lin, Chih-Chan Hseu, You-Cheng Hung, Shih-Ting Sun, Ding-Ping Lin, Kai-Yuan
Contributors:	Asia Univ Hosp, Dept Surg Asia Univ, Dept Biotechnol Tainan Municipal An Nan Hosp, Dept Surg Taipei Med Univ, Sch Med, Dept Pathol, Coll Med Taipei Med Univ, Wan Fang Hosp, Dept Pathol Chi Mei Med Ctr, Dept Med Res China Med Univ, Dept Cosmeceut Asia Univ, Dept Hlth & Nutr Biotechnol Chi Mei Med Ctr, Dept Surg Chia Nan Univ Pharm & Sci, Dept Food Sci & Technol Chia Nan Univ Pharm & Sci, Dept Biotechnol
Keywords:	CERS6 gastric cancer immunohistochemistry prognosis SOCS2
Date:	2018-12
Issue Date:	2019-11-15 15:48:56 (UTC+8)
Publisher:	WILEY
Abstract:	Although gastric cancer (GC) is one of the most common cancers, knowledge of its development, and carcinogenesis is limited. The present study explored the involvement of ceramide synthase 6 (CERS6) in GC carcinogenesis and prognosis. RT-PCR, immunoblotting, and immunohistochemistry were used to examine the expression of CERS6. Transfection and small hairpin RNA technology were used to investigate the effect of CERS6 manipulation on cell proliferation and spread as well as the underlying mechanism. Moreover, xenograft proliferation was employed to explore the influence of CERS6 on tumor growth in animals. It was found that overexpression of CERS6 was significantly correlated with several clinicopathologic parameters and poor disease-free survival. The overexpression and silencing of CERS6 in GC cells facilitated and suppressed cell proliferation and spread as well as xenograft proliferation, respectively. Mechanistic studies further revealed that CERS6 influenced cell proliferation and spread by regulating cell cycle control and metastasis-related protein through the SOCS2/JAK2/STAT3 signaling pathway. Collectively, this study suggests that CERS6 overexpression could be a useful biomarker for predicting the outcomes of GC patients and that CERS6 targeting represents a potential modality for treating GC.
???metadata.dc.relation.uri???:	http://dx.doi.org/10.1002/mc.22888
Relation:	Molecular Nutrition & Food Research, v.57, n.12, pp.1675-1689
Appears in Collections:	[Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles [Dept. of Biotechnology (including master's program)] Periodical Articles

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