LRWD1 Regulates Microtubule Nucleation and Proper Cell Cycle Progression in the Human Testicular Embryonic Carcinoma Cells

doi:10.1002/jcb.26180

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Title:	LRWD1 Regulates Microtubule Nucleation and Proper Cell Cycle Progression in the Human Testicular Embryonic Carcinoma Cells
Authors:	Wang, Chia-Yih Hong, Yu-Han Syu, Jhih-Siang Tsai, Yung-Chieh Liu, Xiu-Ying Chen, Ting-Yu Su, Yin-Mei Kuo, Pao-Lin Lin, Yung-Ming Teng, Yen-Ni
Contributors:	Natl Cheng Kung Univ, Dept Cell Biol & Anat, Coll Med Natl Cheng Kung Univ, Inst Basic Med Sci, Coll Med Natl Univ Tainan, Dept Biol Sci & Technol Chi Mei Med Ctr, Dept Obstet & Gynecol Taipei Med Univ, Dept Med Chia Nan Univ Pharm & Sci, Dept Sport Management Natl Cheng Kung Univ, Dept Obstet & Gynecol, Coll Med Natl Cheng Kung Univ, Dept Urol, Coll Med
Keywords:	LRWD1 CENTROSOME CELL CYCLE MICROTUBULE NUCLEATION AUTOPHAGY
Date:	2018-01
Issue Date:	2019-11-15 15:48:29 (UTC+8)
Publisher:	WILEY
Abstract:	Leucine-rich repeats and WD repeat domain containing protein 1 (LRWD1) is a testis-specific protein that mainly expressed in the sperm neck where centrosome is located. By using microarray analysis, LRWD1 is identified as a putative gene that involved in spermatogenesis. However, its role in human male germ cell development has not been extensively studied. When checking in the semen of patients with asthenozoospermia, teratozoospermia, and asthenoteratozoospermia, the level of LRWD1 in the sperm neck was significantly reduced with a defective neck or tail. When checking the sub-cellular localization of LRWD1 in the cells, we found that LRWD1 resided in the centrosome and its centrosomal residency was independent of microtubule transportation in NT2/D1, the human testicular embryonic carcinoma, cell line. Depletion of LRWD1 did not induce centrosome re-duplication but inhibited microtubule nucleation. In addition, the G1 arrest were observed in LRWD1 deficient NT2/D1 cells. Upon LRWD1 depletion, the levels of cyclin E, A, and phosphorylated CDK2, were reduced. Overexpression of LRWD1 promoted cell proliferation in NT2/D1, HeLa, and 239T cell lines. In addition, we also observed that autophagy was activated in LRWD1 deficient cells and inhibition of autophagy by chloroquine or bafilomycin A1 promoted cell death when LRWD1 was depleted. Thus, we found a novel function of LRWD1 in controlling microtubule nucleation and cell cycle progression in the human testicular embryonic carcinoma cells. J. Cell. Biochem. 119: 314-326, 2018. (c) 2017 Wiley Periodicals, Inc.
???metadata.dc.relation.uri???:	http://dx.doi.org/10.1002/jcb.26180
Relation:	Journal of Cellular Biochemistry, v.119, n.1, pp.314-326
Appears in Collections:	[Dept. of Sports Management] Periodical Articles

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