Manufacture and characterization of anti-inflammatory liposomes from jumbo flying squid (Dosidicus gigas) skin phospholipid extraction

doi:10.1039/c8fo00767e

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Title:	Manufacture and characterization of anti-inflammatory liposomes from jumbo flying squid (Dosidicus gigas) skin phospholipid extraction
Authors:	Kao, Yi-Feng Wu, Yi-Hsieng Samuel Chou, Chung-Hsi Fu, Shih-Guei Liu, Cheng-Wei Chai, Huey-Jine Chen, Yi-Chen
Contributors:	Council Agr, Seafood Technol Div, Fisheries Res Inst Natl Taiwan Univ, Dept Anim Sci & Technol Natl Taiwan Univ, Sch Vet Med Natl Taiwan Univ, Zoonoses Res Ctr Chia Nan Univ Pharm & Sci, Dept Appl Life Sci & Hlth MingDao Univ, Dept Post Modern Agr
Keywords:	Apoptotic Cells Phosphatidylserine Liposomes Macrophages Involvement Activation Beta Rats Inflammation Modulation Clearance
Date:	2018-07-01
Issue Date:	2019-11-15 15:47:39 (UTC+8)
Publisher:	ROYAL SOC CHEMISTRY
Abstract:	The anti-inflammation properties of marine phospholipids enriched with n-3 fatty acids contribute to anti-inflammatory and inflammation-resolving mediators. Functional squid-skin (SQ) liposomes were manufactured from squid-skin phospholipids, and their anti-inflammatory effects were investigated. SQ liposomes included phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylcholine (PC), and lysophosphatidylcholine (Lyso-PC), and had an approximate diameter of 100 mm. When RAW264.7 cells were treated with the SQ liposome, no (p > 0.05) cytotoxicity was observed below a concentration of 7.5 mg mL(-1). An SQ-liposome pretreatment of lipopolysaccharide (LPS)-induced RAW 264.7 cells showed decreased (p < 0.05) prostaglandin E2 (PGE2), nitric oxide (NO), interleukin-1beta (IL-1), IL-6, and tumor necrosis factor-alpha (TNF-). The engulfment of SQ liposomes by the RAW264.7 cells resulted in lower (p < 0.05) LPS-induced intracellular levels of reactive oxygen species. Furthermore, an SQ-liposome administration ameliorated (p < 0.05) carrageenan-induced paw edema in mice. SQ liposomes may act via apoptotic mimicry to elicit the resolution of inflammation and prevent chronic inflammation-related diseases.
???metadata.dc.relation.uri???:	http://dx.doi.org/10.1039/c8fo00767e
Relation:	Integrative Cancer Therapies, v.9, n.7, pp.3986-3996
Appears in Collections:	[Dept. of Life and Health Science] Periodical Articles

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