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| 標題: | Transcriptional responses of Acropora hyacinthus embryo under the benzo(a)pyrene stress by deep sequencing |
| 作者: | Xiao, Rong
Zhou, Hailong
Chen, Chien-Min
Cheng, Huamin
Li, Hongwu
Xie, Jia
Zhao, Hongwei
Han, Qian
Diao, Xiaoping |
| 貢獻者: | Hainan Univ, State Key Lab Marine Resource Utilizat South Chin
Hainan Univ, Inst Trop Agr & Forestry
Hainan Univ, Coll Ocean
Chia Nan Univ Pharm & Sci, Dept Environm Resources Management
Hainan Normal Univ, Key Lab Trop Isl Ecol, Minist Educ |
| 關鍵字: | Acropora hyacinthus embryo
Benzo(a)pyrene
Deep sequencing
Simple Sequence Repeats
Single nucleotide Polymorphisms |
| 日期: | 2018-09 |
| 上傳時間: | 2019-11-15 15:47:19 (UTC+8) |
| 出版者: | PERGAMON-ELSEVIER SCIENCE LTD |
| 摘要: | Coral embryos are a critical and sensitive period for the early growth and development of coral. Benzo(a) pyrene (BaP) is widely distributed in the ocean and has strong toxicity, but there is little information on the toxic effects to coral embryos exposed to this widespread environmental contaminant. Thus, in this study, we utilized the Illumina Hiseg(TM) 4000 platform to explore the gene response of Acropora hyacinthus embryos under the BaP stress. A total of 130,042 Unigenes were obtained and analyzed, and approximately 37.67% of those matched with sequences from four different species. In total, 2606 Unigenes were up-regulated, and 3872 Unigenes were down-regulated. After Gene Ontology (GO) annotation, the results show that the "cellular process" and "metabolic process" were leading in the category of biological processes, which the "binding" and "catalytic activity" were the most abundant subcategories in molecular function. Based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, the most differentially expressed genes (DEGs) were enriched, as well as down-regulated in the pathways of oxidative phosphorylation, metabolism of xenobiotics, immune-related genes, apoptosis and human disease genes. At the same time, 388,197 of Single-nucleotide Polymorphisms (SNPs) and 6164 of Simple Sequence Repeats (SSRs) were obtained, which can be served as the richer and more valuable SSRs molecular markers in the future. The results of this study can help to better understand the toxicological mechanism of coral embryo exposed to BaP, and it is also essential for the protection and restoration of coral reef ecosystem in the future. (C) 2018 Elsevier Ltd. All rights reserved. |
| link: | http://dx.doi.org/10.1016/j.chemosphere.2018.04.149 |
| 關聯: | Clinical Science, v.206, pp.387-397 |
| 顯示於類別: | [環境資源管理系(所)] 期刊論文
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