Production of New Isoflavone Glucosides from Glycosylation of 8-Hydroxydaidzein by Glycosyltransferase from Bacillus subtilis ATCC 6633

doi:10.3390/catal8090387

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 生物科技系(所) > 期刊論文 > Item 310902800/32249

請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/32249

標題:	Production of New Isoflavone Glucosides from Glycosylation of 8-Hydroxydaidzein by Glycosyltransferase from Bacillus subtilis ATCC 6633
作者:	Chiang, Chien-Min Wang, Tzi-Yuan Yang, Szu-Yi Wu, Jiumn-Yih Chang, Te-Sheng
貢獻者:	Chia Nan Univ Pharm & Sci, Dept Biotechnol Acad Sinica, Biodivers Res Ctr Natl Univ Tainan, Dept Biol Sci & Technol Natl Quemoy Univ, Dept Food Sci
關鍵字:	Bacillus glycosyltransferase 8-hydroxydaidzein
日期:	2018-09
上傳時間:	2019-11-15 15:46:47 (UTC+8)
出版者:	MDPI
摘要:	8-Hydroxydaidzein (8-OHDe) has been proven to possess some important bioactivities; however, the low aqueous solubility and stability of 8-OHDe limit its pharmaceutical and cosmeceutical applications. The present study focuses on glycosylation of 8-OHDe to improve its drawbacks in solubility and stability. According to the results of phylogenetic analysis with several identified flavonoid-catalyzing glycosyltransferases (GTs), three glycosyltransferase genes (BsGT110, BsGT292 and BsGT296) from the genome of the Bacillus subtilis ATCC 6633 strain were cloned and expressed in Escherichia coli. The three BsGTs were then purified and the glycosylation activity determined toward 8-OHDe. The results showed that only BsGT110 possesses glycosylation activity. The glycosylated metabolites were then isolated with preparative high-performance liquid chromatography and identified as two new isoflavone glucosides, 8-OHDe-7-O-beta-glucoside and8-OHDe-8-O-beta-glucoside, whose identity was confirmed by mass spectrometry and nuclear magnetic resonance spectroscopy. The aqueous solubility of 8-OHDe-7-O-beta-glucoside and 8-OHDe-8-O-beta-glucoside is 9.0- and 4.9-fold, respectively, higher than that of 8-OHDe. Moreover, more than 90% of the initial concentration of the two 8-OHDe glucoside derivatives remained after 96 h of incubation in 50 mM of Tris buffer at pH 8.0. In contrast, the concentration of 8-OHDe decreased to 0.8% of the initial concentration after 96 h of incubation. The two new isoflavone glucosides might have potential in pharmaceutical and cosmeceutical applications.
link:	http://dx.doi.org/10.3390/catal8090387
關聯:	Indian Journal of Dermatology Venereology & Leprology, v.8, n.9, 387
顯示於類別:	[生物科技系(所)] 期刊論文

文件中的檔案:

檔案	描述	大小	格式	瀏覽次數
10.3390-catal8090387.pdf		3930Kb	Adobe PDF	407	檢視/開啟
index.html		0Kb	HTML	1237	檢視/開啟

在CNU IR中所有的資料項目都受到原著作權保護.

TAIR相關文章

資料載入中.....