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    標題: Sequential Biotransformation of Antcin K by Bacillus subtilis ATCC 6633
    作者: Chang, Te-Sheng
    Chiang, Chien-Min
    Siao, Yi-Yun
    Wu, Jiumn-Yih
    貢獻者: Natl Univ Tainan, Dept Biol Sci & Technol
    Chia Nan Univ Pharm & Sci, Dept Biotechnol
    Natl Quemoy Univ, Dept Food Sci
    關鍵字: Antrodia cinnamomea
    antcin K
    Bacillus subtilis
    biotransformation
    glycosylation
    日期: 2018-09
    上傳時間: 2019-11-15 15:46:44 (UTC+8)
    出版者: MDPI
    摘要: The biotransformation of antcin K, a major ergostane triterpenoid from the fruiting bodies of Antrodia cinnamomea, by Bacillus subtilis (B. subtilis) ATCC 6633 was studied. Four metabolites from the biotransformation were isolated with preparative high-performance liquid chromatography and identified as 25S-antcin K 26-O-beta-glucoside, 25R-antcin K 26-O-beta-glucoside, 25S-antcin K 26-O-beta-(6'-O-succinyl)-glucoside, and 25R-antcin K 26-O-beta-(6'-O-succinyl)-glucoside with mass and nuclear magnetic resonance spectral analysis. By using either 25S-antcin K 26-O-beta-glucoside or 25R-antcin K 26-O-beta-glucoside as the biotransformation precursor, it was proven that 25S-antcin K 26-O-beta-(6'-O-succinyl)-glucoside and 25R-antcin K 26-O-beta-(6'-O-succinyl)-glucoside were biotransformed from 25S-antcin K 26-O-beta-glucoside and 25R-antcin K 26-O-beta-glucoside, respectively. To the best of our knowledge, this is the first study on the glycosylation of triterpenoids from A. cinnamomea, and the first time the succinylation of triterpenoid glycosides by microorganisms has been found. In addition, all four antcin K glucoside derivatives are new compounds.
    link: http://dx.doi.org/10.3390/catal8090349
    關聯: Catalysts, v.8, n.9, 349
    顯示於類別:[生物科技系(所)] 期刊論文

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