Pterostilbene Attenuates Hexavalent Chromium-Induced Allergic Contact Dermatitis by Preventing Cell Apoptosis and Inhibiting IL-1 beta-Related NLRP3 Inflammasome Activation

doi:10.3390/jcm7120489

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Title:	Pterostilbene Attenuates Hexavalent Chromium-Induced Allergic Contact Dermatitis by Preventing Cell Apoptosis and Inhibiting IL-1 beta-Related NLRP3 Inflammasome Activation
Authors:	Wang, Bour-Jr Chiu, Hui-Wen Lee, Yong-Lin Li, Chia-Yi Wang, Ying-Jan Lee, Yu-Hsuan
Contributors:	Chia Nan Univ Pharm & Sci, Dept Cosmet Sci Chia Nan Univ Pharm & Sci, Inst Cosmet Sci Natl Cheng Kung Univ Hosp, Dept Occupat & Environm Med Taipei Med Univ, Shuang Ho Hosp, Dept Internal Med, Div Nephrol Taipei Med Univ, Coll Med, Grad Inst Clin Med Natl Cheng Kung Univ, Coll Med, Dept Environm & Occupat Hlth Univ Alberta, Honors Neurosci Neurosci & Mental Hlth Inst, Fac Sci China Med Univ, China Med Univ Hosp, Dept Med Res Natl Cheng Kung Univ, Coll Med, Dept Food Safety Hyg & Risk Management
Keywords:	hexavalent chromium pterostilbene endoplasmic reticulum stress inflammatory cytokines NLRP3 inflammasome allergic contact dermatitis
Date:	2018-12
Issue Date:	2019-11-15 15:46:33 (UTC+8)
Publisher:	MDPI
Abstract:	Hexavalent chromium (Cr(VI)) is widely used in many industries but can induce contact dermatitis especially in cement industries. Many cement workers suffer from Cr(VI)-induced allergic contact dermatitis (ACD), and prevention and therapeutic strategies are still lacking. Pterostilbene (PT) is a natural compound predominantly found in blueberries. Studies indicate the potential use of PT as an effective anti-oxidative and anti-inflammatory agent. Herein, we investigated the possible mechanisms involved and whether chromium-induced ACD could be effectively inhibited by treating PT. In our in vivo study, epidermal Cr(VI) administration causes cutaneous inflammation in mice ear skin, and the pro-inflammatory cytokines, TNF-alpha, and IL-1 beta, were found in the epidermis, presenting the level of increase after Cr(VI) treatment. Meanwhile, the results of our in vitro experiment showed that apoptosis and endoplasmic reticulum (ER) stress were induced after treatment with different concentrations of Cr(VI) in HaCaT cells (human keratinocyte). Cr(VI) also induced TNF-alpha and IL-beta mRNA expressions, through the activation of the p38 mitogen-activated protein kinase (MAPK)/MAPK-activated protein kinase 2 (MK2) pathway. Notably, the severity of the skin reactions in the epicutaneous elicitation test significantly diminished when the mouse was treated with PT. Likewise, PT intervention also ameliorated the inflammation and apoptosis of HaCaT cells in vitro. Furthermore, our current findings demonstrated that the NLRP3 inflammasome could be involved in the Cr(VI)-mediated inflammation and apoptosis of ACD. Thus, interrupting this mechanism with proper nontoxic agents, such as PT, could be a new option to improve occupational chromium toxicity and hypersensitivity.
???metadata.dc.relation.uri???:	http://dx.doi.org/10.3390/jcm7120489
Relation:	Bmc Pregnancy and Childbirth, v.7, n.12, 489
Appears in Collections:	[Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles

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