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    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/32235

    標題: Uridine Diphosphate-Dependent Glycosyltransferases from Bacillus subtilis ATCC 6633 Catalyze the 15-O-Glycosylation of Ganoderic Acid A
    作者: Chang, Te-Sheng
    Wu, Jiumn-Yih
    Wang, Tzi-Yuan
    Wu, Kun-Yuan
    Chiang, Chien-Min
    貢獻者: Natl Univ Tainan, Dept Biol Sci & Technol
    Natl Quemoy Univ, Dept Food Sci
    Acad Sinica, Biodivers Res Ctr
    Chia Nan Univ Pharm & Sci, Dept Biotechnol
    關鍵字: ganoderic acid
    Bacillus subtilis
    日期: 2018-11
    上傳時間: 2019-11-15 15:46:11 (UTC+8)
    出版者: MDPI
    摘要: Bacillus subtilis ATCC (American type culture collection) 6633 was found to biotransform ganoderic acid A (GAA), which is a major lanostane triterpenoid from the medicinal fungus Ganoderma lucidum. Five glycosyltransferase family 1 (GT1) genes of this bacterium, including two uridine diphosphate-dependent glycosyltransferase (UGT) genes, BsUGT398 and BsUGT489, were cloned and overexpressed in Escherichia coli. Ultra-performance liquid chromatography confirmed the two purified UGT proteins biotransform ganoderic acid A into a metabolite, while the other three purified GT1 proteins cannot biotransform GAA. The optimal enzyme activities of BsUGT398 and BsUGT489 were at pH 8.0 with 10 mM of magnesium or calcium ion. In addition, no candidates showed biotransformation activity toward antcin K, which is a major ergostane triterpenoid from the fruiting bodies of Antrodia cinnamomea. One biotransformed metabolite from each BsUGT enzyme was then isolated with preparative high-performance liquid chromatography. The isolated metabolite from each BsUGT was identified as ganoderic acid A-15-O-beta-glucoside by mass and nuclear magnetic resonance spectroscopy. The two BsUGTs in the present study are the first identified enzymes that catalyze the 15-O-glycosylation of triterpenoids.
    link: http://dx.doi.org/10.3390/ijms19113469
    關聯: International Journal of Molecular Sciences, v.19, n.11, 3469
    Appears in Collections:[生物科技系(所)] 期刊論文

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