Capping Actin Protein Overexpression in Human Colorectal Carcinoma and Its Contributed Tumor Migration

doi:10.1155/2018/8623937

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標題:	Capping Actin Protein Overexpression in Human Colorectal Carcinoma and Its Contributed Tumor Migration
作者:	Tsai, Tsung-Jung Lim, Yun-Ping Chao, Wen-Ying Chen, Chien-Chin Chen, Yi-Ju Lin, Ching-Yen Lee, Ying-Ray
貢獻者:	Chiayi Christian Hosp, Dept Gastroenterol, Ditmanson Med Fdn China Med Univ, Coll Pharm, Dept Pharm Min Hwei Coll Hlth Care Management, Dept Nursing Chiayi Christian Hosp, Ditmanson Med Fdn, Dept Pathol Chia Nan Univ Pharm & Sci, Dept Cosmet Sci Chiayi Christian Hosp, Ditmanson Med Fdn, Dept Med Res
關鍵字:	Gelsolin Cancer Capg Adenocarcinoma Progression Cells
日期:	2018
上傳時間:	2019-11-15 15:45:36 (UTC+8)
出版者:	HINDAWI LTD
摘要:	Objective. Human colorectal cancer (CRC) is the third most common cancer; patients with metastatic colorectal cancer (mCRC) show poor prognosis than those with CRC cases. There are no reliable molecular biomarkers for the diagnosis of CRC prognosis except with pathological features. Therefore, it is urgent to develop a biomarker for diagnosis and/or prediction of human CRC. In addition, capping actin protein (CapG) belongs to the gelsolin family and has been reported to contribute on tumor invasion/metastasis in multiple human cancers. Here, we are the first to evaluate the expression of CapG in human CRCs. Study Design. To investigate the expression levels of CapG in human tissue array by immunohistochemistry (IHC) staining. Moreover, the mRNA and protein levels were also confirmed in four CRC cell lines and determined using real-time RT-PCR and Western blotting. Finally, a Matrigel transwell invasion assay was used to evaluate the invasion ability in CapG high or low expression cells. Results. We demonstrated that CapG could be determined in the normal colon tissue and human CRC specimens. However, CapG was significantly overexpressed in the mCRC specimens compared with that in CRC specimens and normal cases. It was also detectable in the four CRC cell lines including mRNA and protein levels. We also found that knockdown of the expression of CapG reduced tumor migration. Conclusions. In this study, we suggested that CapG could be used as a biomarker for metastatic CRC in the clinical specimens. Moreover, our in vitro study demonstrated that CapG might contribute on tumor metastasis in human CRCs.
link:	http://dx.doi.org/10.1155/2018/8623937
關聯:	Biomed Research International, v.2018, ID 8623937
顯示於類別:	[化妝品應用與管理系(所)] 期刊論文

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