Raspberry ketone induces brown-like adipocyte formation through suppression of autophagy in adipocytes and adipose tissue

doi:10.1016/j.jnutbio.2018.01.017

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Title:	Raspberry ketone induces brown-like adipocyte formation through suppression of autophagy in adipocytes and adipose tissue
Authors:	Leu, Sy-Ying Tsai, Yung-Chieh Chen, Wen-Chi Hsu, Chih-Hsiung Lee, Yen-Mei Cheng, Pao-Yun
Contributors:	Natl Def Med Ctr, Grad Inst Life Sci Chi Mei Med Ctr, Dept Obstet & Gynecol Taipei Med Univ, Dept Med Chia Nan Univ Pharm & Sci, Dept Sport Management Natl Def Med Ctr, Grad Inst Physiol, Dept Physiol & Biophys Natl Def Med Ctr, Triserv Gen Hosp, Dept Internal Med Natl Def Med Ctr, Dept Pharmacol
Keywords:	Raspberry ketone 3T3-L1 Adipocyte Ovariectomy Obesity Beige adipocyte
Date:	2018-06
Issue Date:	2019-11-15 15:44:59 (UTC+8)
Publisher:	ELSEVIER SCIENCE INC
Abstract:	Promoting white adipose tissue (WAT) to acquire brown-like characteristics is a promising approach for obesity treatment. Although raspberry ketone (RK) has been reported to possess antiobesity activity, its effects on the formation of brown-like adipocytes remain unclear. Therefore, we investigated the effects and underlying mechanism of RK on WAT browning in 313-L1 adipocytes and rats with ovariectomy (Ovx)-induced obesity. RK (100 mu M) significantly induced browning of 3T3-L1 cells by increasing mitochondrial biogenesis and the expression of browning-specific proteins (PR domain containing 16, PRDM16; peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PGC-1 alpha; uncoupling protein-1, UCP-1) and lipolytic enzymes (hormone-sensitive lipase and adipose triglyceride lipase). RK significantly reduced the expression of the autophagy-related protein Atg12 and increased the expression of p62 and heme oxygenase 1 (1-10-1). Additionally, these effects of RK were reversed by the HO-1 inhibitor SnPP (20 mu M). In addition, RK (160 mg/kg, gavage, for 8 weeks) significantly reduced body weight gain (Ovx+ RK, 191.8 + 4.6 g vs. Ovx, 223.6 +/- 5.9; P < .05), food intake, the amount of inguinal adipose tissue (Ovx+ RK, 9.05 +/- 1.1 g vs Ovx, 12.9 +/- 0.92 g; P < .05) and the size of white adipocytes in Ovx rats. Moreover, compared to expression in the Ovx group, the levels of browning-specific proteins were significantly higher and the levels of autophagy-related proteins were significantly lower in the Ovx+RK group. Therefore, this study elucidated the mechanism associated with RK-induced WAT browning and thus provides evidence to support the clinical use of RK for obesity treatment. (C) 2018 Elsevier Inc. All rights reserved.
???metadata.dc.relation.uri???:	http://dx.doi.org/10.1016/j.jnutbio.2018.01.017
Relation:	World Neurosurgery, v.56, pp.116-125
Appears in Collections:	[Dept. of Sports Management] Periodical Articles

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