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https://ir.cnu.edu.tw/handle/310902800/32182
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| 標題: | Naked physically synthesized gold nanoparticles affect migration, mitochondrial activity, and proliferation of vascular smooth muscle cells |
| 作者: | Lo, Huey-Ming
Ma, Ming-Chieh
Shieh, Jiunn-Min
Chen, Hui-Ling
Wu, Wen-Bin |
| 貢獻者: | Fu Jen Catholic Univ, Sch Med
Shin Kong Wu Ho Su Mem Hosp, Sect Cardiol, Dept Internal Med
Chi Mei Med Ctr, Dept Internal Med
Chia Nan Univ Pharm & Sci, Dept Recreat & Healthcare Management
Fu Jen Catholic Univ, Holist Educ Ctr |
| 關鍵字: | adhesion
AuNP
cardiovascular disease
FAK
platelet-derived growth factor
VSMC
TEM |
| 日期: | 2018 |
| 上傳時間: | 2019-11-15 15:44:03 (UTC+8) |
| 出版者: | DOVE MEDICAL PRESS LTD |
| 摘要: | Introduction: Vascular smooth muscle cells (VSMCs) play an important role in the development and progression of atherosclerosis and vascular injuries in terms of proliferation and migration. Therefore, the aim of this study was to investigate the anti-migratory and proliferative effects of naked gold nanoparticles (AuNPs) on VSMCs. Materials and methods: One set of physically synthesized AuNPs (pAuNPs) and three sets of chemically synthesized AuNPs (cAuNPs) were tested. Results and discussion: Among them, the pAuNPs were found to significantly and markedly inhibit platelet-derived growth factor (PDGF)-induced VSMC migration. Transmission electron microscopy revealed that the pAuNPs were ingested and aggregated in the cytoplasm at an early stage of treatment, while the viability of VSMCs was not affected within 24 hours of treatment. The pAuNP treatment enhanced cellular mitochondrial activity but inhibited basal and PDGF-induced VSMC proliferation, as determined by MTT, WST-1, and BrdU cell proliferation assays. Furthermore, the pAuNPs did not interfere with PDGF signaling or matrix metalloproteinase-2 expression/activity. Unlike the cAuNPs, the pAuNPs could markedly reduce VSMC adhesion to collagen, which was supported by the findings that the pAuNPs could inhibit collagen-induced tyrosine protein and focal adhesion kinase (FAK) phosphorylation and actin cytoskeleton reorganization during cell adhesion. The in vitro effects of the pAuNPs were confirmed in the in vivo rat balloon-injured carotid artery model by diminishing the proliferating VSMCs. Conclusion: Taken together, the present study provides the first evidence that naked pAuNPs can reduce VSMC migration and compromise cell adhesion by affecting FAK and tyrosine-protein activation. The pAuNPs also have an inhibitory effect on PDGF-induced VSMC proliferation and can reduce proliferating/migrating VSMC expression in vivo. |
| link: | http://dx.doi.org/10.2147/IJN.S156880 |
| 關聯: | American Journal of Cancer Research, v.13, pp.3163-3176 |
| 顯示於類別: | [休閒保健管理系(所)] 期刊論文
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