The clinical significance of silent mutations with respect to ciprofloxacin resistance in MRSA

doi:10.2147/IDR.S159455

CNU IR > Pharmacy and Science > Dept. of Health and Nutrition (including master's program) > Periodical Articles > Item 310902800/32179

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/32179

Title:	The clinical significance of silent mutations with respect to ciprofloxacin resistance in MRSA
Authors:	Lai, Chih-Cheng Chen, Chi-Chung Lu, Ying-Chen Chuang, Yin-Ching Tang, Hung-Jen
Keywords:	MRSA silent mutation rpoB474 ciprofloxacin resistance
Date:	2018
Issue Date:	2019-11-15 15:43:57 (UTC+8)
Publisher:	DOVE MEDICAL PRESS LTD
Abstract:	Background: The aim of this study was to investigate the genotypic differences between different sequence type MRSA isolates, especially focusing on silent rpoB474 mutations and the relationship between such mutations and ciprofloxacin resistance. Methods: Seventy-nine MRSA isolates were obtained for antibiotic susceptibility tests and molecular study. Results: Among these isolates, we found that the MIC 50, MIC 90, and minimum inhibitory concentration (MIC) range of ciprofloxacin were much higher for the isolates without the rpoB474 mutation than for isolates with the rpoB474 mutation. A total of 87.5% of the isolates with the rpoB474 mutation were susceptible to ciprofloxacin, but none of the isolates without the rpoB474 mutation were susceptible to ciprofloxacin. For 27 MRSA isolates without rpo474 silent mutation but with gyrA86/126 silent mutation, all of them belonged to SCCmec III, and had high ciprofloxacin MIC levels. For another 44 MRSA isolates with rpo474 silent mutation but without gyrA86/126 silent mutation, all of them showed low ciprofloxacin MIC levels, all of them belonged to either SCCmec IV or V. Furthermore, MRSA ciprofloxacin resistance was found to be associated with the mutations gyrA S84L/parC S80F or gyrA S84L, and S85P/parC S80Y. Conclusion: Most occurrences of this rpoB474 silent mutation were found in community acquired-MRSA (CA-MRSA) isolates with susceptibility to most antibiotics, especially for ciprofloxacin and vice versa. Thus, this mutation may help to differentiate the different microbiologic characteristics of MRSA clinical isolates.
???metadata.dc.relation.uri???:	http://dx.doi.org/10.2147/IDR.S159455
Relation:	Diabetes Metabolic Syndrome and Obesity-Targets and Therapy, v.11, pp.681-687
Appears in Collections:	[Dept. of Health and Nutrition (including master's program)] Periodical Articles

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