Corylin inhibits LPS-induced inflammatory response and attenuates the activation of NLRP3 inflammasome in microglia

doi:10.1186/s12906-018-2287-5

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Title:	Corylin inhibits LPS-induced inflammatory response and attenuates the activation of NLRP3 inflammasome in microglia
Authors:	Huang, Ming-Yii Tu, Chia-En Wang, Shu-Chi Hung, Yung-Li Su, Chia-Cheng Fang, Shih-Hua Chen, Chi-Shuo Liu, Po-Len Cheng, Wei-Chung Huang, Yu-Wei Li, Chia-Yang
Contributors:	Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Radiat Oncol, Ctr Canc Kaohsiung Med Univ, Grad Inst Med, Coll Med Kaohsiung Med Univ, Dept Med Lab Sci & Biotechnol Juntendo Univ, Inst Hlth & Sports Sci & Med Chi Mei Med Ctr, Div Urol, Dept Surg Chia Nan Univ Pharm & Sci, Dept Senior Citizen Serv Management Natl Taiwan Univ Sport, Inst Athlet Natl Tsing Hua Univ, Dept Biomed Engn & Environm Sci Kaohsiung Med Univ, Dept Resp Therapy, Coll Med China Med Univ, Grad Inst Biomed Sci, Res Ctr Tumor Med Sci China Med Univ, Drug Dev Ctr Kaohsiung Chang Gung Mem Hosp, Dept Radiat Oncol
Keywords:	Microglia Corylin MAPK signaling pathway NLRP3 inflammasome Anti-inflammation
Date:	2018-08-15
Issue Date:	2019-11-15 15:43:30 (UTC+8)
Publisher:	BMC
Abstract:	Background: Inflammation has been found to be associated with many neurodegenerative diseases, including Parkinson's and dementia. Attenuation of microglia-induced inflammation is a strategy that impedes the progression of neurodegenerative diseases. Methods: We used lipopolysaccharide (LPS) to simulate murine microglia cells (BV2 cells) as an experimental model to mimic the inflammatory environment in the brain. In addition, we examined the anti-inflammatory ability of corylin, a main compound isolated from Psoralea corylifolia L that is commonly used in Chinese herbal medicine. The production of nitric oxide (NO) by LPS-activated BV2 cells was measured using Griess reaction. The secretion of proinflammatory cytokines including tumor necrosis factor (TNF-alpha), interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) by LPS-activated BV2 cells was analyzed using enzyme-linked immunosorbent assay (ELISA). The expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3 (NLRP3), apoptosis-associated speck-like protein containing a caspase-activation and recruitment domain (ASC), caspase-1, IL-1 beta and mitogen-activated protein kinases (MAPKs) in LPS-activated BV2 cells was examined by Western blot. Results: Our experimental results demonstrated that corylin suppressed the production of NO and proinflammatory cytokines by LPS-activated BV2 cells. In addition, corylin inhibited the expression of iNOS and COX-2, attenuated the phosphorylation of ERK, JNK and p38, decreased the expression of NLRP3 and ASC, and repressed the activation of caspase-1 and IL-1 beta by LPS-activated BV2 cells. Conclusion: Our results indicate the anti-inflammatory effects of corylin acted through attenuating LPS-induced inflammation and inhibiting the activation of NLRP3 inflammasome in LPS-activated BV2 cells. These results suggest that corylin might have potential in treating brain inflammation and attenuating the progression of neurodegeneration diseases.
???metadata.dc.relation.uri???:	http://dx.doi.org/10.1186/s12906-018-2287-5
Relation:	Bmc Psychiatry, v.18, 221
Appears in Collections:	[Dept. of Senior Service and Health Management] Periodical Articles

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