協力劑對埃及斑蚊幼蟲表現解毒作用的影響

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Dissertations and Theses > Item 310902800/31969

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Title:	協力劑對埃及斑蚊幼蟲表現解毒作用的影響 Role of synergists on insecticide detoxification in Aedes aegypti (Diptera:?Culicidae) larvae
Authors:	張箴毅
Contributors:	生物科技系羅怡珮
Keywords:	埃及斑蚊協力劑百滅寧抗性酵素活性解毒基因 Aedes aegypti Synergist Permethrin Resistance Enzyme activity Detoxification genes
Date:	2018
Issue Date:	2019-02-27 16:50:21 (UTC+8)
Abstract:	埃及斑蚊 (Aedes aegypti) 是台灣地區傳播登革熱的主要病媒蚊之一。本研究以 PBO、DEM 及 TPP 三種協力劑處理對百滅寧具高度抗性的埃及斑蚊幼蟲，探討協力劑對埃及斑蚊幼蟲表現解毒作用的影響。以生物檢測法測定百滅寧添加協力劑對埃及斑蚊幼蟲的協力效果，結果顯示百滅寧與 PBO 以 1:3 混合稀釋藥液對埃及斑蚊幼蟲的SR50的值是 7.53，百滅寧與DEM及TPP以 1:6混合稀釋藥液對埃及斑蚊幼蟲的SR50的值分別是 2.27 及 1.07。以協力劑處理埃及斑蚊 4 小時，探討協力劑對幼蟲解毒酵素和解毒基因的影響。試驗結果顯示 PBO 對單加氧?具顯著抑制效果 (p< 0.05)，DEM 對 α-酯?具顯著抑制效果 (p< 0.05)，TPP對 α-酯?及 β-酯?具極顯著的抑制效果 (p< 0.001)。而 PBO 處理埃及斑蚊幼蟲，會提升與殺蟲劑抗性相關之CYP6BB2、CYP6CB1-2、GSTe7等基因的表現量，對CYP9J26基因表現量不具差異性，會下調 CCEae3a 基因的表現量。DEM 處理埃及斑蚊幼蟲會下調與殺蟲劑抗性相關之CYP6BB2、CYP6CB1-2、CYP9J26、CCEae3a及GSTe7等基因的表現量。TPP 處理埃及斑蚊幼蟲會上調與殺蟲劑抗性相關之CYP6BB2基因的表現量，會下調CYP6CB1-2、CYP9J26、CCEae3a及GSTe7基因的表現量。 Aedes aegypti is one of the main vector mosquitoes that transmit dengue fever in Taiwan. The purpose of the study was to investigate the effects of synergists (PBO, DEM and TPP) on the detoxification of larvae. The synergistic effect of Permethrin on the larvae of Aedes aegypti was determined by bioassay. The results showed that the SR50 value of PBO against Aedes aegypti larvae was 7.53, followed by DEM (2.27) and TPP (1.13). The insecticide effect of LYPR larvae after synergists treated 4 hours were the optimum. After 4 hours pretreated Aedes aegypti larvae with synergists, the results showed that PBO had a significant inhibitory effect on monooxygenase activity (p<0.05), DEM had a significant inhibitory effect on α-esterase activity (p<0.05) and TPP had a significant inhibitory effect on α-esterase and β-esterase activity (p<0.001). After 4 hours pretreated Aedes aegypti larvae with synergists, the results showed that PBO upregulated the gene expression of CYP6BB2, CYP6CB1-2, CYP9J26 and GSTe7, and TPP upregulated the gene expression of CYP6BB2. The other genes expression pretreated with synergists were all down regulation.
Relation:	電子全文公開日期：2021-09-07，學年度：106， 51頁
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Dissertations and Theses

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