室溫離子液體是一種可經由陽離子與陰離子的變化，而產生多種組合之特性來應用在各分析化學中各個領域，如電化學分析、層析分析，對於化學分析研究都是一種很好的溶劑與材料。本研究利用不同的離子液體添加在微透析的灌流液中，來改善體外微透析相對回收率差的問題，近而有效提升分析物的相對回收率。並以添加離子液體會影響微透析的回收率來作探討，結果顯示導入離子液體於微透析的技術中，能夠顯著地提升抗癲癇藥物之相對回收率。 In the clinical treatment of Antiepileptic drug, patients with multiple epilepsy types sometimes need two or more antiepileptic drugs, so the relationship between the effect of antiepileptic treatment and blood concentration has a monitoring value. However, this study utilizes microdialysis to measure the characteristics of microdialysis sampling in living extracellular fluid sampling techniques, as well as in anesthetized or awake animal blood and various organs. Decided to use microdialysis technology as a sampling method for antiepileptic drugs; now microdialysis technology combined with high performance liquid chromatography instrument can obtain qualitative and quantitative determination of drugs, and is also widely used in basic and clinical neuromedical research. in. Find a high-performance liquid chromatography method to detect microdialysis samples and simultaneously analyze and quantify the microdialysis samples of antiepileptic drugs.The samples were separated on HPLC by using C-18 (25cm×4.6mm i.d., 5μm) column and mobile phase of acetonitrile:methanol:phosphate buffer(pH 6.0) (10:28:62 (v/v)) in 1.0mL/min flow rate. All drugs were monitored and quantified by UV 210nm. Six antiepileptic drugs can be separated simultaneously within 45 minutes and quantified.
However, due to other characteristics such as high lipophilicity of the antiepileptic drug itself, the relative recovery rate of microdialysis is low, and the quantification of the analyte is relatively difficult.Room temperature ionic liquid is a kind of solvent which can be applied to various fields of analytical chemistry, such as electrochemical analysis and chromatographic analysis, through the change of cation and anion, and produces various combinations. It is a good solvent for chemical analysis. With materials. In this study, different ionic liquids were added to the microdialysis perfusion solution to improve the relative recovery rate of microdialysis in vitro, and the relative recovery rate of the analyte was improved. The addition of ionic liquids will affect the recovery rate of microdialysis. The results show that the introduction of ionic liquids in microdialysis technology can significantly improve the relative recovery of antiepileptic drugs.
