丹參活性成分及細胞毒性分析

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標題:	丹參活性成分及細胞毒性分析 Analysis of active components and cytotoxicity of Salvia miltiorrhiza
作者:	李雅雲
貢獻者:	保健營養系林翠品
關鍵字:	丹參丹參酮 IIA 丹參酚酸 B Salvia miltiorrhiza tanshinone IIA salvianolic acid B
日期:	2018
上傳時間:	2019-02-27 16:45:16 (UTC+8)
摘要:	丹參(Salvia miltiorrhiza)，為唇形科(Lamiaceae)植物，其根部主要含有親酯性的丹參酮及親水性的丹參酚酸衍生物。其中，丹參酮IIA及丹參酚酸B各為抗癌、保護心血管、降低血糖及抗氧化的活性指標成分。本研究探討本土種植丹參，丹參酚酸 B 與丹參酮IIA含量以及抗淋巴腫瘤細胞功效。以高效能液相層析法分析，丹參乙醇及水萃取物，所含的丹參酮 IIA 及丹參酚酸 B 的含量；以 MTT 方法測試丹參酮 IIA 、丹參酚酸 B 以及丹參萃取物對淋巴腫瘤細胞存活率的影響。結果顯示，丹參乙醇萃取物與水萃取物中丹參酚酸 B含量均大於5%以上，以丹參70%乙醇萃取物 (SmE70)中丹參酚酸 B含量最高，約8.6%。而丹參乙醇萃取物中丹參酮 IIA含量大於0.2%以上，以丹參70%及95%乙醇萃取物 (SmE70及SmE95) 含量最高，約0.6%，丹參水萃取物(SmW)只有0.03%。在 MTT 試驗中，丹參酚酸B 對細胞沒有毒性，丹參酮 IIA 在濃度0.5 g / ml 以上對淋巴腫瘤細胞具有毒性；在萃取物中，SmE50 、SmE70及 SmE95 在濃度50 g/ml時影響淋巴腫瘤細胞存活率，約79.3%、 55.6%及59.5%；而 SmW 對細胞不具有毒性作用。 Salvia miltiorrhiza is a plant belonging to the family Lamiaceae. Its roots mainly contain the lipophilic tanshinone and hydrophilic salvianolic acid derivatives. Among them, tanshinone IIA and salvianolic acid B are index compounds of anti-cancer, cardiovascular protection, lower blood glucose and antioxidant activity. This study was to explore the salvianolic acid B and tanshinone IIA levels and anti-tumor activity of the local planting, S. miltiorrhiza local planted. The contents of tanshinone IIA and salvianolic acid B in the ethanolic and aqueous extracts from S. miltiorrhiza were determined by high performance liquid chromatography analysis. Effects of tanshinone IIA, salvianolic acid B and the ethanolic and aqueous extracts from S. miltiorrhiza on cell viability were measured by MTT method. The results showed that the contents of salvianolic acid B in the ethanolic and aqueous extracts from S. miltiorrhiza were more than 5%, and the 70% ethanolic extract from S.miltiorrhiza (SmE70) contained the highest content of salvianolic acid B, about 8.6%. Furthermore, the contents of tanshinone IIA were more than 0.2% in the ethanolic extracts from S. miltiorrhiza. The contents of 70% and 95% ethanolic extracts from S. miltiorrhiza (SmE70 and SmE95) were the highest, about 0.6%, while the content of the aqueous extracts from S. miltiorrhiza (SmW) was only 0.03%. In the MTT assay, salvianolic acid B did not affect cells viability, and tanshinone IIA was toxic to lymphoma cells at a concentration of 0.5 g / ml. These extracts from S. miltiorrhiza, SmE50, SmE70 and SmE95 at 50 g / ml were toxic to lymphoma cells, the survival rates were about 79.3%, 55.6% and 59.5%, respectively; however, SmW had no toxic to the cells.
關聯:	電子全文公開日期：2023-02-22，學年度：106， 65頁
显示于类别:	[保健營養系(所) ] 博碩士論文

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