English  |  正體中文  |  简体中文  |  Items with full text/Total items : 17744/20032 (89%)
Visitors : 7298339      Online Users : 267
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/31761

    標題: MMP1 expression is activated by Slug and enhances multi-drug resistance (MDR) in breast cancer
    作者: Shen, Ching-Ju
    Kuo, Yu-Ling
    Chen, Chien-Chung
    Chen, Ming-Jenn
    Cheng, Ya-Min
    貢獻者: Kaohsiung Med Univ, Grad Inst Med
    Kaohsiung Med Univ,Kaohsiung Med Univ Hosp, Dept Gynecol & Obstet
    E Da Hosp, Dept Plast & Reconstruct Surg
    Chi Mei Med Ctr, Dept Surg
    Chia Nan Univ Pharm & Sci, Dept Sports Management
    Natl Cheng Kung Univ, Inst Clin Med, Dept Obstet & Gynecol
    關鍵字: Matrix-Metalloproteinase
    Mesenchymal Transition
    Signaling Pathway
    日期: 2017-03-23
    上傳時間: 2018-11-30 15:55:42 (UTC+8)
    出版者: Public Library Science
    摘要: High matrix metalloproteinase 1 (MMP1) expression is associated with enhanced breast cancer growth and metastasis and also might predict poor prognosis. In this study, we further investigated the functional role of MMP1 and how it is upregulated in multi-drug resistant (MDR) breast cancer cells. By retrieving microarray data in GEO datasets and the survival data in the Kaplan Meier plotter, we observed that MMP1 is significantly upregulated in MCF-7/ADR cells compared to the parental MCF-7 cells, while high MMP1 expression is associated with worse overall survival (OS) and recurrence free survival (RFS) in breast cancer patients after systematic therapy. Functional studies showed that MMP1 overexpression significantly reduced the drug sensitivity in MCF-7 cells, while MMP1 knockdown substantially enhanced the sensitivity in MCF-7/ADR cells. By performing western blotting and immunofluorescent staining, we confirmed that MCF-7/ADR cells had enhanced mesenchymal properties than MCF-7 cells. In MCF-7 cells, enforced Slug expression resulted in significant MMP1 upregulation, while in MCF-7/ADR cells, Slug knockdown led to reduced MMP1 expression. By performing bioinformatic analysis, we observed that the promoter of MMP1 has three putative Slug binding sites. The following dual luciferase assay and ChIP-qPCR verified these three binding sites. Therefore, we infer that Slug enhances MMP1 transcription via directly binding to the promoter region in breast cancer cells, which is a previously unrecognized mechanism in the development of MDR.
    關聯: Plos One, v.12, n.3, e0174487
    Appears in Collections:[運動管理系] 期刊論文

    Files in This Item:

    File Description SizeFormat
    10.1371-journal.pone.0174487.pdf3708KbAdobe PDF0View/Open

    All items in CNU IR are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback