Heme oxygenase-1 mediates anti-adipogenesis effect of raspberry ketone in 3T3-L1 cells

doi:10.1016/j.phymed.2017.05.005

CNU IR > College of Sustainable Environment and Recreation > Dept. of Sports Management > Periodical Articles > Item 310902800/31754

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Title:	Heme oxygenase-1 mediates anti-adipogenesis effect of raspberry ketone in 3T3-L1 cells
Authors:	Tsai, Yung-Chieh Yang, Bo-Cheng Peng, Wen-Huang Lee, Yen-Mei Yen, Mao-Hsiung Cheng, Pao-Yun
Contributors:	Chi Mei Med Ctr, Dept Obstet & Gynaecol Taipei Med Univ, Dept Med Chia Nan Univ Pharm & Sci, Dept Sport Management China Med Univ, Dept Chinese Pharmaceut Sci & Chinese Med Resourc Natl Def Med Ctr, Dept Pharmacol Natl Def Med Ctr, Dept Physiol & Biophys
Keywords:	Raspberry ketone Heme oxygenase-1 beta-catenin Adipogenesis Obesity
Date:	2017-07-15
Issue Date:	2018-11-30 15:55:26 (UTC+8)
Publisher:	Elsevier Gmbh, Urban & Fischer Verlag
Abstract:	Background: Obesity is caused by excessive accumulation of body fat and is closely related to complex metabolic diseases. Raspberry ketone (RK), a major aromatic compound in red raspberry, was recently reported to possess anti-obesity effects. However, its mechanisms are unclear. Aim: Adipogenesis plays a critical role in obesity and, therefore, this study aimed to investigate the effect and mechanisms of action of RK on adipogenesis in 3T3-L1 preadipocytes. Materials and methods: 3T3-L1 preadipocytes were differentiated in medium containing insulin, dexamethasone, and 1-methyl-3-isobutylxanthine. Adipocyte lipid contents were determined using oil-red O staining while adipogenic transcription factor and lipogenic protein expressions were determined using western blotting. Results: RK (300-400 mu M) strongly inhibited lipid accumulation during 3T3-L1 preadipocyte differentiation into adipocytes. RK reduced the CCAAT/enhancer-binding protein-a (C/EBP-a), peroxisome proliferation-activated receptor-gamma (PPAR-gamma), fatty acid synthase (FAS), and fatty acid-binding protein 4 (FABP4) expressions and increased heme oxygenase-1 (HO-1), Wnt10b, and beta-catenin expressions in 3T3-L1 adipocytes. Additionally, RK inhibited lipid accumulation, and adipogenic transcription factor and lipogenic protein expressions were all decreased by inhibiting HO-1 or beta-catenin using tin protoporphyrin (SnPP) or beta-catenin short-interfering RNA (siRNA), respectively. Furthermore, Wnt10b and beta-catenin expressions were negatively regulation by SnPP. Conclusion: RK may exert anti-adipogenic effects through modulation of the HO-1/Wnt/beta-catenin signaling pathway.
Relation:	Phytomedicine, v.31, pp.11-17
Appears in Collections:	[Dept. of Sports Management] Periodical Articles

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