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| 標題: | Argininosuccinate lyase interacts with cyclin A2 in cytoplasm and modulates growth of liver tumor cells |
| 作者: | Hung, Yu-Hsuan
Huang, Hau-Lun
Chen, Wei-Ching
Yen, Meng-Chi
Cho, Chien-Yu
Weng, Tzu-Yang
Wang, Chih-Yang
Chen, Yi-Ling
Chen, Li-Tzong
Lai, Ming-Derg |
| 貢獻者: | Natl Cheng Kung Univ, Coll Med, Inst Basic Med Sci
Natl Cheng Kung Univ, Coll Med, Dept Biochem & Mol Biol
Natl Cheng Kung Univ, Coll Med, Ctr Infect Dis & Signaling Res
Natl Hlth Res Inst, Natl Inst Canc Res
Kaohsiung Med Univ,Kaohsiung Med Univ Hosp, Dept Emergency Med
Chia Nan Univ Pharm & Sci, Dept Senior Citizen Serv Management
Natl Hlth Res Inst, Natl Inst Canc Res |
| 關鍵字: | argininosuccinate lyase
liver cancer
non-enzymatic function
cyclin A2
drug resistance
arginine deiminase |
| 日期: | 2017-02 |
| 上傳時間: | 2018-11-30 15:55:00 (UTC+8) |
| 出版者: | Spandidos Publ Ltd |
| 摘要: | Arginine is a critical amino acid in specific cancer types including hepatocellular carcinoma (HCC) and melanoma. Novel molecular mechanisms and therapeutic targets in arginine metabolism-mediated cancer formation await further identification. Our laboratory has previously demonstrated that arginine metabolic enzyme argininosuccinate lyase (ASL) promoted HCC formation in part via maintenance of cyclin A2 protein expression and arginine production for channeling to nitric oxide synthase. In this study, we investigated the mechanism by which ASL regulates cyclin A2 expression. We found that ASL interacted with cyclin A2 in HCC cells and the localization of their interaction was in the cytoplasm. Mutation of essential residues for enzymatic activity of ASL did not affect the binding of ASL to cyclin A2. Moreover, the mutant ASL retained the ability to restore the decreased tumorigenicity caused by ASL shRNA. Furthermore, overexpression of ASL conferred resistance to arginine deprivation therapy. Finally, the important pathways and potential therapeutic targets in ASL-regulated HCC were identified by bioinformatics analyses with Metacore database and Connectivity Map database. Our analyses suggested that bisoprolol, celecoxib, and ipratropium bromide, are potential therapeutics for ASL-regulated HCC formation. Thus, ASL interacts with cyclin A2 in cytoplasm, and may promote HCC formation through this non-enzymatic function. Overexpression of ASL may be a contributing factor in drug resistance for arginine deprivation therapy. |
| 關聯: | Oncology Reports, v.37, n.2, pp.969-978 |
| 顯示於類別: | [高齡福祉養生管理系] 期刊論文
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