Chitosan promotes immune responses, ameliorating total mature white blood cell numbers, but increases glutamic oxaloacetic transaminase and glutamic pyruvic transaminase, and ameliorates lactate dehydrogenase levels in leukemia mice in vivo

doi:10.3892/mmr.2017.6923

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Title:	Chitosan promotes immune responses, ameliorating total mature white blood cell numbers, but increases glutamic oxaloacetic transaminase and glutamic pyruvic transaminase, and ameliorates lactate dehydrogenase levels in leukemia mice in vivo
Authors:	Yeh, Ming-Yang Shih-, Yung-Luen Chung, Hsueh-Yu Chou, Jason Lu, Hsu-Feng Liu, Chia-Hui Liu, Jia-You Huang, Wen-Wen Peng, Shu-Fen Wu, Lung-Yuan Chung, Jing-Gung
Contributors:	Cheng Hsin Gen Hosp, Off Director Fu Jen Catholic Univ, Dept Sch Med Shin Kong Wu Ho Su Mem Hosp, Dept Pathol & Lab Med Taipei Med Univ, Sch Med Lab Sci & Biotechnol Jen Teh Jr Coll Med Nursing & Management Cheng Hsin Gen Hosp, Dept Anat Pathol Cheng Hsin Gen Hosp, Dept Clin Pathol Chia Nan Univ Pharm & Sci, Ctr Gen Educ China Med Univ, Dept Biol Sci & Technol I Shou Univ, Sch Chinese Med Postbaccalaureate Asia Univ, Dept Biotechnol
Keywords:	chitosan immune response glutamic oxaloacetic transaminase glutamic pyruvic transaminase lactate dehydrogenase
Date:	2017-09
Issue Date:	2018-11-30 15:54:25 (UTC+8)
Publisher:	Spandidos Publ Ltd
Abstract:	The aim of the present study was to investigate the effect of chitosan (a naturally derived polymer) on the immune responses and glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) and lactate dehydrogenase (LDH) levels in WEHI-3 cell-generated leukemia mice. Mice were divided into control, WEHI-3 control, acetic acid (vehicle) -treated, and 5 and 20 mg/kg chitosan-treated groups. Mice were subsequently weighed, blood was collected, and liver and spleen samples were isolated and weighed. Blood samples were measured for cell markers, the spleen underwent phagocytosis and natural killer (NK) cell activity examination, and cell proliferation was analyzed by flow cytometry. Chitosan did not significantly affect the weights of body, liver and spleen at 5 and 20 mg/kg treatment. Chitosan increased the percentage of CD3 (T cells marker), decreased the levels of CD19 (B-cell marker) and CD11b at 5 mg/kg treatment, and decreased the levels of Mac-3 at 5 and 20 mg/kg treatment. Chitosan significantly increased macrophage phagocytosis of PBMCs, but did not significantly affect macrophage phagocytosis in the peritoneal cavity. Chitosan treatment did not significantly affect the cytotoxic activity of NK cells, and also did not affect T-and B-cell proliferation. Chitosan significantly increased total white blood cell numbers, and GOT and GPT activities were both significantly increased. However, chitosan did not significantly affect LDH activity in leukemia mice. Chitosan may aid in future studies on improving immune responses in the treatment of leukemia.
Relation:	Molecular Medicine Reports, v.16, n.3, pp.2483-2490
Appears in Collections:	[The Center For General Education] Periodical Articles

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