Raspberry Ketone Reduced Lipid Accumulation in 3T3-L1 Cells and Ovariectomy-Induced Obesity in Wistar Rats by Regulating Autophagy Mechanisms

doi:10.1021/acs.jafc.7b03831

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Title:	Raspberry Ketone Reduced Lipid Accumulation in 3T3-L1 Cells and Ovariectomy-Induced Obesity in Wistar Rats by Regulating Autophagy Mechanisms
Authors:	Leu, Sy-Ying Chen, Yi-Chen Tsai, Yung-Chieh Hung, Yao-Wen Hsu, Chih-Hsiung Lee, Yen-Mei Cheng, Pao-Yun
Contributors:	Natl Def Med Ctr, Grad Inst Life Sci Natl Def Med Ctr, Grad Inst Physiol, Dept Physiol & Biophys Chi Mei Med Ctr, Dept Obstet & Gynecol Taipei Med Univ, Dept Med Chia Nan Univ Pharm & Sci, Dept Sport Management Natl Def Med Ctr, Inst Prevent Med Triserv Gen Hosp,Natl Def Med Ctr, Dept Internal Med Natl Def Med Ctr, Dept Pharmacol
Keywords:	raspberry ketone autophagy adipogenesis ovariectomy obesity
Date:	2017-12-20
Issue Date:	2018-11-30 15:52:38 (UTC+8)
Publisher:	Amer Chemical Soc
Abstract:	This study aimed to determine the antiobesity effects of raspberry ketone (RK), one of the major aromatic compounds contained in raspberry, and its underlying mechanisms. During adipogenesis of 3T3-L1 cells, RK (300 mu M) significantly reduced lipid accumulation and downregulated the expression of CCAAT/enhancer-binding protein alpha (C/EBP alpha), peroxisome proliferation-activated receptor gamma (PPAR gamma), fatty acid-binding protein 4 (FABP4), and fatty acid synthase (FAS). RK also reduced the expression of light chain 3B (LC3B), autophagy-related protein 12 (Atg12), sirtuin 1 (SIRT1), and phosphorylated-tuberous sclerosis complex 2 (TSC2), whereas it increased the level of p62 and phosphorylated-mammalian target of rapamycin (mTOR). Daily administration of RK decreased the body weight (ovariectomy [Ovx] + RK, 352.6 +/- 5 vs Ovx, 386 +/- 5.8 g; P < 0:05), fat mass (Ovx + RK, 3.2 +/- 0.05 vs Ovx, 5.0 +/- 0.4 g; P < 0.05), and fat cell size (Ovx + RK, 6.4 +/- 0.6 vs Ovx, 11.1 +/- 0.7 X 10(3) mu m(2); P < 0.05) in Ovx-induced obesity in rats. The expression of PPAR gamma, C/EBP alpha, FAS, and FABP4 was significantly reduced in the Ovx + RK group compared with that in the Ovx group. Similar patterns were observed in autophagy-related proteins and endoplasmic reticulum stress proteins. These results suggest that RK inhibited lipid accumulation by regulating autophagy in 3T3-L1 cells and Ovx-induced obese rats.
Relation:	Journal of Agricultural and Food Chemistry, v.65, n.50, pp.10907-10914
Appears in Collections:	[Dept. of Sports Management] Periodical Articles

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