Induction of apurinic endonuclease 1 overexpression by endoplasmic reticulum stress in hepatoma cells

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Title:	Induction of apurinic endonuclease 1 overexpression by endoplasmic reticulum stress in hepatoma cells
Authors:	Wu(吳明軒), Ming-Syuan Cheng(鄭琮霖), Tsung-Lin Huang(黃國泰), Kuo-Tai Huang(黃國權), Cuo-Chiuan Zhang(張家維), Jia-Wei Hung(洪瑞祥), Jui-Hsiang
Contributors:	Department of Physiology, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan Department of Biotechnology, Chia Nan University of Pharmacy and Science, Tainan , Taiwan
Date:	2015-05-05
Issue Date:	2018-03-27 10:44:15 (UTC+8)
Abstract:	Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide with poor prognosis due to resistance to conventional chemotherapy and limited efficacy of radiotherapy. Previous study have indicated that induction of endoplasmic reticulum stress or apurinic endonuclease 1 (APE1)were observedin many tumors. Therefore, the aim of this study was to investigate therelationship between endoplasmic reticulum (ER stress) and APE1 in hepatocellular carcinoma.Here we show that the expression of APE1during ER stress in HepG2 and Huh-7 celllines. Tunicamycin or brefeldin A, two ER stress inducers, increased APE1 and GRP78, ER stress marker, expression in HepG2 and Huh-7 cells. Induction of APE1 expression was through transcription level in response to ER stress. We found APE1nuclear localization during ER stress by using immunofluorescence assay in HepG2 cells. Furthermore, expression of Hepatitis B virus pre-S2? large mutant surface protein (pre-S2?), ER stress-induced protein, also increased GRP78 and APE1 expression in normal hepatocyte NeHepLxHT cell line. Similarly, tumor sample showed higher expression of APE1 in ER stress-correlated liver cancer tissue in vivo.Our result demonstrates that ER stress and HBV pre-S2? increased APE1expression whichmay plays an important role in resistance to chemotherapeuticagents or tumor development. Therefore, these data provide an important chemotherapeutic strategy in ER stress and HBV pre-S2?-associated tumor.
Relation:	2015 第九屆嘉南藥理大學藥理學院師生研究成果發表會，起迄日：2015/05/05-2015/05/08，地點：嘉南藥理大學國際會議中心一樓
Appears in Collections:	[Pharmacy and Science] 2015

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