IL-1α regulated the induction of proinflammatory cytokine expression in cancer-associated fibroblasts

CNU IR > Pharmacy and Science > 2015 > Item 310902800/31238

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Title:	IL-1α regulated the induction of proinflammatory cytokine expression in cancer-associated fibroblasts
Authors:	WANG(王秀華), HSIU-HUA (田孝威), SHIAW-WEI TYAN
Contributors:	Department of Biotechnology, Chia Nan University of Pharmacy and Science Drug Discovery and Development Center, Chia Nan University ofPharmacy and Science
Keywords:	breast cancer cell fibroblast IL-1 proinflammatory cytokine
Date:	2015-05-05
Issue Date:	2018-03-27 10:41:06 (UTC+8)
Abstract:	Cancer-associated fibroblasts (CAFs) have been shown to secrete high levels of proinflammatorycytokines. These proinflammatory cytokines, such asinterleukin-6 (IL-6) and IL-8, were reported to assistcancer cell proliferation, angiogenesis and invasion. However, the mechanisms by which fibroblasts become proinflammatory ones during cancer progression are still not known. Here, we showed that through co-culturing with breast cancer cells for at least three-four passages, breast normal tissue-associated fibroblasts (NAFs) obtained persistent activity for expressing high levels of proinflammatory factors, including IL-1, IL-1, IL-6 and IL-8. IL-1and IL-1were respectively reported to play a role in induction of IL-6 and IL-8, so we tested the hypothesis that breast cancer cells might induce IL-6 and IL-8 expression in NAFs via anIL-1or IL-1signaling pathway. By using an IL-1neutralizing antibody, we found that abolishment ofIL-1activity completely repressed the enhanced IL-8 expression and partially reduced the enhanced IL-1and IL-6 expression in NAFs that were pre-cocultured with breast cancer MDA-MB-468 cells. However, an IL-1neutralizing antibody had no effect on the expression of these cytokines. Furthermore, the treatment of an IL-1neutralizing antibody also reduced the enhanced IL-1, IL-6 and IL-8 expression in CAFs. These results suggested that IL-1may mediate induction of IL-1, IL-6 and IL-8 expression in cancer-associated fibroblasts. AnIL-1signaling pathway in stromalcells may be a target for prevention of breast cancer development and progression.
Relation:	2015 第九屆嘉南藥理大學藥理學院師生研究成果發表會，起迄日：2015/05/05-2015/05/08，地點：嘉南藥理大學國際會議中心一樓
Appears in Collections:	[Pharmacy and Science] 2015

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