The role of COX-2 and COX-derived prostaglandins in breast cancer cell-induced ADAMTS1 expression in fibroblasts

CNU IR > Pharmacy and Science > 2015 > Item 310902800/31237

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Title:	The role of COX-2 and COX-derived prostaglandins in breast cancer cell-induced ADAMTS1 expression in fibroblasts
Authors:	Hsu(許朝凱), Chao-Kai (田孝威), Shiaw-Wei Tyan
Contributors:	Department of Biotechnology, Chia Nan University of Pharmacy and Science,Tainan 71710, Taiwan, R.O.C. (嘉南藥理大學生物科技系) Drug Discovery and Development Center, Chia Nan University ofPharmacy and Science, Tainan 71710, Taiwan, R.O.C. (嘉南藥理大學新藥創建研究中心)
Keywords:	breast cancer cell fibroblast COX-2 prostaglandin ADAMTS1
Date:	2015-05-05
Issue Date:	2018-03-27 10:40:55 (UTC+8)
Abstract:	Our previous study showed that breast cancer-associated fibroblasts (CAFs) expressed high level of ADAM metallopeptidase withthrombospondin type 1 motif, 1(ADAMTS1)to increase invasion of cancer cells. Through co-culturing with breast cancer cells for at least four passages, breast normal tissue-associated fibroblasts (NAFs) obtained persistent activityfor promoting cancer cell invasion via enhancing ADAMTS1 expression to the identical level of CAFs. ADAMTS1 has been shown to be up-regulated by cyclooxygenase (COX)-derived prostaglandin E2(PGE2)and prostaglandin F2(PGF2), so we tested the hypothesis that breast cancer cells might induce ADAMTS1 expression in NAFs via a COX/prostaglandin signaling pathway.By using a COX-2 inhibitor NS398, we found that inhibition of COX-2 activity completely repressed the enhanced ADAMTS1 expression in NAFsthat were co-cultured with breast cancer MDA-MB-468 cells. Inhibition of COX-2 activity also reduced the ability of breast cancer cell-cocultured NAFs to promote cancer cell invasion.AH6809, an antagonist for EP1and EP2receptors (PGE2receptors), and AL8810, an antagonist for a FP receptor (a PGF2receptor), both exhibited ~70% of repression on the enhanced ADAMTS1 expression in breast cancer cell-cocultured NAFs. The results indicated that other COX-downstream signaling pathways might also induce ADAMTS1 expression. Furthermore, the treatment of a COX-2 inhibitor did not decrease the ADAMTS1 mRNA level in CAFs, which might be due to irreversible change of fibroblasts once induced by cancer cells. Our results suggested that breast cancer cells may induced ADAMTS1 expression in stromal fibroblasts via a COX/prostaglandin signaling pathwayto facilitate cancer cell invasion. The COX/prostaglandin pathway in stromalcells may be a target for prevention of breast cancer progression.
Relation:	2015 第九屆嘉南藥理大學藥理學院師生研究成果發表會，起迄日：2015/05/05-2015/05/08，地點：嘉南藥理大學國際會議中心一樓
Appears in Collections:	[Pharmacy and Science] 2015

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