Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/31156
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 18054/20253 (89%)
造访人次 : 24250857      在线人数 : 564
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/31156


    標題: Lercanidipine and labedipinedilol-A play anti-inflammatory role through inhibition of lipopolysaccharide/interferon-γ-induced HMGB1 release and MMP-2, 9 activities in vascular smooth muscle cells
    作者: Shu-Fen Liou(劉淑芬)
    Ling-Cheng Kao(高翎程)
    貢獻者: Department and Graduate Institute of Pharmacology, College of Medicine, Kaohsiung Medical University
    日期: 2015-05-05
    上傳時間: 2018-03-27 10:24:14 (UTC+8)
    摘要: Inflammation is an important molecular basis of atherosclerosis. Recent studies have shown that dihydropyridine calcium channel blockers (CCBs) can exert potent anti-inflammatory effects in models of vascular dysfunction. The purpose of the present study was to evaluate anti-inflammatory effects and mechanisms of lercanidipine and labedipinedilol-A, new generation dihydropyridine CCBs, in rat vascular smooth muscle cells (VSMCs) exposed to lipopolysaccharide (LPS) and interferon-γ (IFN-γ). MTT, Griess reagent, RT-PCR, ELISA, gelatin zymography, immunocytochemistry and Western blotting were employed. We found that lercanidipine and labedipinedilol-A attenuated production of NO, ROS, TNF-α and IL- from LPS/IFN-γ-stimulated VSMCs. In addition, they both diminished the LPS/IFN-γ-induced expression of iNOS protein and mRNA, with attenuation of HMGB1 cytosolic translocation and subsequent extracellular release.
    Furthermore, they down-regulated MMP-2/MMP-9 activities, while expression of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1), an inhibitor of MMP-9, was up-regulated. Finally, we found that lercanidipine and labedipinedilol-A inhibited the nuclear translocation of NF-κB and suppressed the phosphorylation of JNK, p38 MAPK and Akt. In conclusion, lercanidipine and labedipinedilol-A can exert their anti-inflammatory effects through suppression of NO, ROS, TNF-α and IL- through down-regulation of iNOS, MMP-2/MMP-9, and HMGB1, with inhibition of signaling transduction of MAPKs, Akt/IkB-α and NF-κB pathways. These findings implicate a valuable role of new generation dihydropyridine CCBs lercanidipine and labedipinedilol-A inthe treatment of inflammatory vascular diseases.
    關聯: 2015 第九屆嘉南藥理大學藥理學院師生研究成果發表會,起迄日:2015/05/05-2015/05/08,地點:嘉南藥理大學國際會議中心一樓
    显示于类别:[藥理學院] 2015 第九屆嘉南藥理大學藥理學院師生研究成果發表會

    文件中的档案:

    档案 描述 大小格式浏览次数
    B03.pdf595KbAdobe PDF241检视/开启


    在CNU IR中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈