Investigation of β-glucuronidase inhibitor from the secondary metabolites of the root of Neolitsea acuminatissima

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Title:	Investigation of β-glucuronidase inhibitor from the secondary metabolites of the root of Neolitsea acuminatissima
Authors:	Hsiao-Jung Chou (周孝容) Chih-Chi Chang(張芷綺) Chu-Hung Lin(林居宏) Ih-Sheng Chen(陳益昇) Hsun-Shuo Chang(張訓碩) Tian-Lu Cheng(鄭添祿) Horng-Huey Ko(柯宏慧)
Contributors:	Department of Fragrance and Cosmetic Science, Kaohsiung Medical University School of Pharmacy, Kaohsiung Medical University Department of Biological Science and Environmental Biology, Kaohsiung Medical University
Keywords:	Neolitsea acuminatssima β-glucuronidase diarrhea
Date:	2015-11-14
Issue Date:	2018-03-27 09:34:01 (UTC+8)
Abstract:	According to literature, the main factor induced diarrhea in chemotherapy is β-glucuronidase of Escherichia coli (eβG). The authors desire to investigate eβG inhibitor, for the release of side-effect induced by chemotherapy. In the preliminary screen data, the methnolic extract of the root of Neolitsea acuminatissima (NA) showed the anti- eβG activity and without affecting human β- glucuronidase (hβG) activity. The results indicated that NA is a valuable source from which natural product-based medicinal products can be derived. In fact, the phytochemistry and the anti-eβG activity of the root of NA has not been investigated. Thus, the root of NA was selected as the candidate. The methanolic extract of NA (NARM) was partitioned with dichloromethane (NARD), ethyl acetate (NARE), n-butanol (NARB), and water (NARW) to obtain different soluble fractions. Bioassay-guided fractionation of the root of NARE and NARD, one new eudesmanolide type sesquiterpenes, methoxyneolitacumone A (8),one new alkaloid, demethoxydaibucarboline A (5), along with 8 known compounds, namely as zeorin (1), neolitacumone C (2), quercetin (3), dihydroquercetin (4), β-sitosterol (6), neolitacumone (7), oplopanone (9), and epicatechin (11), were isolated and the structures were characterized spectroscopically. Among them, compounds 3 and 5 showed potent anti-eβG activity with inhibition ratio of 70-80%.
Relation:	2015台灣藥學年會暨學術研討會，起迄日：2015/11/14，地點：嘉南藥理大學
Appears in Collections:	[Pharmacy and Science] 2015 Annual symposium of the pharmaceutical society of Taiwan

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