The mechanisms of Kaempferol-induces cell apoptosis in HUVEC cells

CNU IR > Pharmacy and Science > 2015 Annual symposium of the pharmaceutical society of Taiwan > Item 310902800/31123

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Title:	The mechanisms of Kaempferol-induces cell apoptosis in HUVEC cells
Authors:	Chiu-Fang Lee Ni-Na Chiang Jai-Sing Yang Yu-Syuan Huang Chun Chen Fu-An Chen
Contributors:	Kaohsiung Veterans General Hospital Pingtung Branch, Pingtung Bracco Pharmaceutial Corp.Ltd,. Department of Pharmacy&Graduate Institute of Pharmaceutical Technology, Tajen University
Keywords:	Kaempferol angiogenesis HUVECs apoptosis ROS MAPK JNK signal
Date:	2015-11-14
Issue Date:	2018-03-27 09:33:49 (UTC+8)
Abstract:	The purpose of this study explored the role of Kaempferol on anti-angiogenic property and the mechanism of human umbilical cell (HUVECs) apoptosis. In this report,Kaempferol dose- and time-dependently inhibited viability of HUVECs. In in vitro study, Kaemferol induced apoptotic death of HUVECs by detecting DNA fragmentation and promoted reactive oxygen species (ROS) production using flow cytometric assay. In addition, the extrinsic caspase singnaling (caspase-8 and caspase-3) were activated in Kaempferol-treated HUVECs, and their inhibitors were applied to check the singnal transduction. We investigated the upstream of death receptor pathway and further observed that the levels of death receptor 5 (DR5) and phosphorylated c-jun N-terminal kinase (JNK) signals were up-regulated in HUVECs aftter Kaempferol challenge, which were confirmed by a JNK specific inhibitor (SO600125). Hence, Kaempferol-induced endothelial cell apoptosis was involved in an ROS/JNK-regulated DR5 pathway. IN summary, Kaempferol might process a potential therapeutic effect for anti-vascular targeting of angiogenesis during cancer treatment.
Relation:	2015台灣藥學年會暨學術研討會，起迄日：2015/11/14，地點：嘉南藥理大學
Appears in Collections:	[Pharmacy and Science] 2015 Annual symposium of the pharmaceutical society of Taiwan

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