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https://ir.cnu.edu.tw/handle/310902800/31060
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| 標題: | OSR1 and SPAK cooperatively modulate Sertoli cell support of mouse spermatogenesis |
| 作者: | Liu, Yung-Liang
Yang, Sung-Sen
Chen, Shyi-Jou
Lin, Yu-Chun
Chu, Chin-Chen
Huang, Hsin-Hui
Chang, Fung-Wei
Yu, Mu-Hsien
Lin, Shih-Hua
Wu, Gwo-Jang
Sytwu, Huey-Kang |
| 貢獻者: | Natl Def Med Ctr, Grad Inst Med Sci
Tri Serv Gen Hosp, Natl Def Med Ctr, Dept Obstet & Gynecol
Tri Serv Gen Hosp, Dept Obstet & Gynecol, Penghu Branch
Tri Serv Gen Hosp, Natl Def Med Ctr, Div Nephrol, Dept Med
Natl Def Med Ctr, Dept & Grad Inst Microbiol & Immunol
Tri Serv Gen Hosp, Dept Pediat, Natl Def Med Ctr
Tri Serv Gen Hosp, Dept Pathol, Natl Def Med Ctr
Chi Mei Med Ctr, Dept Anesthesiol
Chia Nan Univ Pharm & Sci, Dept Recreat & Hlth Care Management
Natl Def Med Ctr, Grad Inst Life Sci |
| 關鍵字: | transcription factor gata-1
alanine-rich kinase
k-cl cotransporter
smooth-muscle tone
blood-pressure
a-myb
na+-k+-2cl(-) cotransporter
tyrosine phosphorylation
mice
gene |
| 日期: | 2016-11 |
| 上傳時間: | 2018-01-18 11:40:50 (UTC+8) |
| 出版者: | Nature Publishing Group |
| 摘要: | We investigated the role of oxidative stress-responsive kinase-1 (OSR1) and STE20 (sterile 20)/SPS1-related proline/alanine-rich kinase (SPAK), upstream regulators of the Na+-K+-2Cl(-) cotransporter (NKCC1)-essential for spermatogenesis-in mouse models of male fertility. Global OSR1(+/-) gene mutations, but not global SPAK(-/-) or Sertoli cell (SC)-specific OSR1 gene knockout (SC-OSR1(-/-)), cause subfertility with impaired sperm function and are associated with reduced abundance of phosphorylated (p)-NKCC1 but increased p-SPAK expression in testicular tissue and spermatozoa. To dissect further in a SC-specific manner the compensatory effect of OSR1 and SPAK in male fertility, we generated SC-OSR1(-/-) and SPAK(-/-) double knockout (DKO) male mice. These are infertile with defective spermatogenesis, presenting a SC-only-like syndrome. Disrupted meiotic progression and increased germ cell apoptosis occurred in the first wave of spermatogenesis. The abundance of total and p-NKCC1 was significantly decreased in the testicular tissues of DKO mice. These results indicate that OSR1 and SPAK cooperatively regulate NKCC1-dependent spermatogenesis in a SC-restricted manner. |
| 關聯: | Scientific Reports, v.6, 37205 |
| 顯示於類別: | [休閒保健管理系(所)] 期刊論文
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