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    標題: Antimicrobial and antitumor activities of chitosan from shiitake stipes, compared to commercial chitosan from crab shells
    作者: Chien, Rao-Chi
    Yen, Ming-Tsung
    Mau, Jeng-Leun
    貢獻者: NCHU, Dept Food Sci & Biotechnol
    NCHU, Plant & Food Biotechnol Ctr, NCHU Univ Calif Davis
    NCHU, Agr Biotechnol Ctr
    Chia Nan Univ Pharm & Sci, Dept Appl Life Sci & Hlth
    關鍵字: Shiitake chitosan
    Crab chitosan
    Antimicrobial
    Antitumor
    Lentinula edodes
    日期: 2016-03
    上傳時間: 2018-01-18 11:40:27 (UTC+8)
    出版者: Elsevier Sci Ltd
    摘要: Chitosan was prepared by alkaline N-deacetylation of chitin obtained from shiitake stipes and crab shells and its antimicrobial and antitumor activities were studied. Chitosan from shiitake stipes and crab shells exhibited excellent antimicrobial activities against eight species of Gram positive and negative pathogenic bacteria with inhibition zones of 11.4-26.8 mm at 0.5 mg/ml. Among chitosan samples, shiitake chitosan C120 was the most effective with inhibition zones of 16.4-26.8 mm at 0.5 mg/ml. In addition, shiitake and crab chitosan showed a moderate anti-proliferative effect on IMR 32 and Hep G2 cells. At 5 mg/ml, the viability of IMR 32 cells incubated with chitosan was 68.8-85.0% whereas that of Hep G2 cells with chitosan was 60.4-82.9%. Overall, shiitake chitosan showed slightly better antimicrobial and antitumor activities than crab chitosan. Based on the results obtained, shiitake and crab chitosan were strong antimicrobial agents and moderate antitumor agents. (C) 2015 Elsevier Ltd. All rights reserved.
    關聯: Carbohydrate Polymers, v.138, pp.259-264
    Appears in Collections:[生活應用與保健系] 期刊論文

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