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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/31015

    標題: Biotransformation of isoflavones daidzein and genistein by recombinant Pichia pastoris expressing membrane-anchoring and reductase fusion chimeric CYP105D7
    作者: Chiang, Chien-Min
    Ding, Hsiou-Yu
    Lu, Jing-Yu
    Chang, Te-Sheng
    貢獻者: Chia Nan Univ Pharm, Dept Biotechnol
    Chia Nan Univ Pharm, Dept Cosmet Sci
    Natl Univ Tainan, Dept Biol Sci & Technol
    關鍵字: Hydroxylation
    Cytochrome P450 monooxygenase
    Pichia pastoris
    Streptomyces avermitilis
    日期: 2016-03
    上傳時間: 2018-01-18 11:39:52 (UTC+8)
    出版者: Elsevier Science Bv
    摘要: The biotransformation of soy isoflavones into ortho-hydroxyisoflavones by CYP105D7 from Streptomyces avermitilis MA4680 is investigated through chimeric expression in Pichia pastoris. Using N-terminal fusion With the transmembrane domain of CYP57B3 from Aspergillus oryzae and C-terminal fusion with a P450 reductase from Saccharomyces cerevisiae, CYP105D7 was expressed in the form of reductase fusion and membrane anchoring in P. pastoris. Recombinant P. pastoris expressing the chimera catalyzed the biotransformation of both daidzein and genistein. This is the first study to show the catalyzing activity of CYP105D7 towards genistein. The major product from daidzein was identified as 6-hydroxydaidzein by comparing the results of the ultra-performance liquid chromatography analysis with the authentic standard. The major product from genistein was purified using preparative high-performance liquid chromatography and identified as 3'-hydroxygenistein based on nuclear magnetic resonance and mass data. The recombinant P. pastoris produced 6-hydroxydaidzein and 3'-hydroxygenistein in a 5-l fermenter, with maximal yields of 7.5 and 15.0 mg/l, respectively. The production of 3'-hydroxygenistein was higher than any previously reported in the literature. (C) 2015 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
    關聯: Journal of the Taiwan Institute of Chemical Engineers, v.60, pp.26-31
    Appears in Collections:[化妝品應用與管理系(所)] 期刊論文
    [生物科技系(所)] 期刊論文

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