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https://ir.cnu.edu.tw/handle/310902800/31011
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| 標題: | Heat shock protein 70 and AMP-activated protein kinase contribute to 17-DMAG-dependent protection against heat stroke |
| 作者: | Tsai, Yung-Chieh
Lam, Kwok-Keung
Peng, Yi-Jen
Lee, Yen-Mei
Yang, Chung-Yu
Tsai, Yi-Ju
Yen, Mao-Hsiung
Cheng, Pao-Yun |
| 貢獻者: | Chi Mei Med Ctr, Dept Obstet & Gynecol
Taipei Med Univ, Dept Med
Chia Nan Univ Pharm & Sci, Dept Sport Management
Taipei Med Univ, Dept Pharmacol
Catholic Mercy Hosp, Dept Anesthesiol
Triserv Gen Hosp, Dept Pathol
Natl Def Med Ctr
Natl Def Med Ctr, Dept Pharmacol
Natl Def Med Ctr, Dept Physiol & Biophys |
| 關鍵字: | heat stroke
heat shock protein 70
AMP-activated protein kinase
17-DMAG
autophagy
mortality |
| 日期: | 2016-10 |
| 上傳時間: | 2018-01-18 11:39:47 (UTC+8) |
| 出版者: | Wiley-Blackwell |
| 摘要: | Heat shock protein 70 (Hsp70) preconditioning induces thermotolerance, and adenosine monophosphate (AMP)-activated protein kinase (AMPK) plays a role in the process of autophagy. Here, we investigated whether 17-dimethylaminoethylamino-17-demethoxy-geldanamycin (17-DMAG) protected against heat stroke (HS) in rats by up-regulation of Hsp70 and phosphorylated AMPK (pAMPK). To produce HS, male Sprague-Dawley rats were placed in a chamber with an ambient temperature of 42 degrees C. Physiological function (mean arterial pressure, heart rate and core temperature), hepatic and intestinal injury, inflammatory mediators and levels of Hsp70, pAMPK and light chain 3 (LC3B) in hepatic tissue were measured in HS rats or/and rats pre-treated with 17-DMAG. 17-DMAG pre-treatment significantly attenuated hypotension and organ dysfunction induced by HS in rats. The survival time during HS was also prolonged by 17-DMAG treatment. Hsp70 expression was increased, whereas pAMPK levels in the liver were significantly decreased in HS rats. Following pre-treatment with 17-DMAG, Hsp70 protein levels increased further, and pAMPK levels were enhanced. Treatment with an AMPK activator significantly increased the LC3BII/LC3BI ratio as a marker of autophagy in HS rats. Treatment with quercetin significantly suppressed Hsp70 and pAMPK levels and reduced the protective effects of 17-DMAG in HS rats. Both of Hsp70 and AMPK are involved in the 17-DMAG-mediated protection against HS. 17-DMAG may be a promising candidate drug in the clinical setting. |
| 關聯: | Journal of Cellular and Molecular Medicine, v.20 n.10, pp.1889-1897 |
| 顯示於類別: | [運動管理系] 期刊論文
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