資料載入中.....
|
請使用永久網址來引用或連結此文件:
https://ir.cnu.edu.tw/handle/310902800/30971
|
| 標題: | Cleome rutidosperma and Euphorbia thymifolia Suppress Inflammatory Response via Upregulation of Phase II Enzymes and Modulation of NF-B and JNK Activation in LPS-Stimulated BV2 Microglia |
| 作者: | Ding, Hsiou-Yu
Wu, Pei-Shan
Wu, Ming-Jiuan |
| 貢獻者: | Chia Nan Univ Pharm & Sci, Dept Cosmet Sci
Chia Nan Univ Pharm & Sci, Dept Biotechnol |
| 關鍵字: | Cleome rutidosperma
Euphorbia thymifolia
microglial cells
HO-1
JNK
NF-B
expression |
| 日期: | 2016-09 |
| 上傳時間: | 2018-01-18 11:38:58 (UTC+8) |
| 出版者: | Mdpi Ag |
| 摘要: | Cleome rutidosperma DC. and Euphorbia thymifolia L. are herbal medicines used in traditional Indian and Chinese medicine to treat various illnesses. Reports document that they have antioxidant and anti-inflammatory activities; nonetheless, the molecular mechanisms involved in their anti-inflammatory actions have not yet been elucidated. The anti-neuroinflammatory activities and underlying mechanisms of ethanol extracts of Cleome rutidosperma (CR) and Euphorbia thymifolia (ET) were studied using lipopolysaccharide (LPS)-stimulated microglial cell line BV2. The morphology changes and production of pro-inflammatory mediators were assayed. Gene expression of inflammatory genes such as inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, interleukin (IL)-1, and CC chemokine ligand (CCL)-2, as well as phase II enzymes such as heme oxygenase (HO)-1, the modifier subunit of glutamate cysteine ligase (GCLM) and NAD(P)H quinone dehydrogenase 1 (NQO1), were further investigated using reverse transcription quantitative-PCR (RT-Q-PCR) and Western blotting. The effects of CR and ET on mitogen activated protein kinases (MAPKs) and nuclear factor (NF)-B signaling pathways were examined using Western blotting and specific inhibitors. CR and ET suppressed BV2 activation, down-regulated iNOS and COX-2 expression and inhibited nitric oxide (NO) overproduction without affecting cell viability. They reduced LPS-mediated tumor necrosis factor (TNF) and IL-6 production, attenuated IL-1 and CCL2 expression, but upregulated HO-1, GCLM and NQO1 expression. They also inhibited p65 NF-B phosphorylation and modulated Jun-N terminal kinase (JNK) activation in BV2 cells. SP600125, the JNK inhibitor, significantly augmented the anti-IL-6 activity of ET. NF-B inhibitor, Bay 11-7082, enhanced the anti-IL-6 effects of both CR and ET. Znpp, a competitive inhibitor of HO-1, attenuated the anti-NO effects of CR and ET. Our results show that CR and ET exhibit anti-neuroinflammatory activities by inhibiting pro-inflammatory mediator expression and production, upregulating HO-1, GCLM and NQO1, blocking NF-B and modulating JNK signaling pathways. They may offer therapeutic potential for suppressing overactivated microglia and alleviating neurodegeneration. |
| 關聯: | International Journal of Molecular Sciences, v.17 n.9, 1420 |
| 顯示於類別: | [生物科技系(所)] 期刊論文
[化妝品應用與管理系(所)] 期刊論文
|
文件中的檔案:
| 檔案 |
描述 |
大小 | 格式 | 瀏覽次數 |
|---|
| 30971.pdf | | 2412Kb | Adobe PDF | 275 | 檢視/開啟 | | index.html | | 0Kb | HTML | 1415 | 檢視/開啟 |
|
在CNU IR中所有的資料項目都受到原著作權保護.
|
