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| 標題: | Chemotherapeutic effects of luteolin on radio-sensitivity enhancement and interleukin-6/signal transducer and activator of transcription 3 signaling repression of oral cancer stem cells |
| 作者: | Tu, Dom-Gene
Lin, Wei-Ting
Yu, Cheng-Chia
Lee, Shiuan-Shinn
Peng, Chih-Yu
Lin, Taichen
Yu, Chuan-Hang |
| 貢獻者: | Ditmanson Med Fdn, Chia Yi Christian Hosp, Dept Nucl Med
Chia Nan Univ Pharm & Sci, Dept Food Sci & Technol
Chang Jung Christian Univ, Coll Hlth Sci
Ditmanson Med Fdn, Chia Yi Christian Hosp, Dept Oral & Maxillofacial Surg
Chung Shan Med Univ, Sch Dent
Chung Shan Med Univ Hosp, Dept Dent
Chung Shan Med Univ, Inst Oral Sci
Chung Shan Med Univ, Sch Publ Hlth |
| 關鍵字: | cancer stem cells
luteolin
oral carcinomas
radio-sensitivity |
| 日期: | 2016-12 |
| 上傳時間: | 2018-01-18 11:38:48 (UTC+8) |
| 出版者: | Elsevier Taiwan |
| 摘要: | Background/Purpose: Previously, we successfully identified oral cancer stem cells (OCSC) displaying enhanced stemness and tumorigenic potentials. In the study, we investigated the chemotherapeutic effect of the flavonoid luteolin, commonly found in fruits and vegetables, on targeting OCSC. Methods: Oralspheres was applied to isolate OCSC. aldehyde dehydrogenase 1 activity and CD44 positivity of OCSC with luteolin treatment were assessed by flow cytometry analysis. Radio-sensitivity of OCSC treated with luteolin was examined. Invasion and colony-forming assays were performed to assess oncogenicity in OCSC. The expression of interleukin-6 (IL-6)/ signal transducer and activator of transcription 3 (STAT3) was examined by enzyme-linked immunosorbent assay and western blot analysis. Results: We showed that luteolin effectively inhibited the proliferation rate, self-renewal, aldehyde dehydrogenase 1 activity, and CD44 positivity of OCSC but did not cause significant cytotoxicity of normal epithelial cells. Moreover, luteolin restored radio-sensitivity in OCSC. Combined treatment with luteolin and radiation displayed synergistic effect on invasiveness and clonogenicity of OCSC. Mechanistically, treatment of luteolin resulted in inactivation of IL-6/STAT3 signaling. Conclusion: These results suggest that combined treatment of luteolin and radiation therapy can attenuate tumorigenicity of OCSC through IL-6/STAT3 signaling inactivation. Copyright (C) 2016, Formosan Medical Association. Published by Elsevier Taiwan LLC. This is an open access article under the CC BY-NC-ND license. |
| 關聯: | Journal of the Formosan Medical Association, v.115 n.12, pp.1032-1038 |
| 顯示於類別: | [ 食品科技系 ] 期刊論文
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