Improving Free Radical Scavenging Activity of Soy Isoflavone Glycosides Daidzin and Genistin by 3 '-Hydroxylation Using Recombinant Escherichia coli

doi:10.3390/molecules21121723

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標題:	Improving Free Radical Scavenging Activity of Soy Isoflavone Glycosides Daidzin and Genistin by 3 '-Hydroxylation Using Recombinant Escherichia coli
作者:	Chiang, Chien-Min Wang, Dong-Sheng Chang, Te-Sheng
貢獻者:	Chia Nan Univ Pharm & Sci, Dept Biotechnol Natl Univ Tainan, Dept Biol Sci & Technol
關鍵字:	antioxidant biotransformation daidzin genistin hydroxylation
日期:	2016-12
上傳時間:	2018-01-18 11:38:29 (UTC+8)
出版者:	Mdpi Ag
摘要:	The present study describes the biotransformation of a commercially available crude extract of soy isoflavones, which contained significant amounts of the soy isoflavone glycosides daidzin and genistin, by recombinant Escherichia coli expressing tyrosinase from Bacillus megaterium. Two major products were isolated from the biotransformation and identified as 3'-hydroxydaidzin and 3'-hydroxygenistin, respectively, based on their mass and nuclear magnetic resonance spectral data. The two 3'-hydroxyisoflavone glycosides showed potent 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity with IC50 values of 7.4 and 9.8 mu M for 3'-hydroxydaidzin and 3'-hydroxygenistin, respectively. The free radical scavenging activities of the two 3'-hydroxyisoflavone glycosides were, respectively, 120 and 72 times higher than the activity of their precursors, daidzin and genistin, and were also stronger than the activity of ascorbic acid, which showed an IC50 value of 15.1 mu M. This is the first report of the bio-production and potential antioxidant applications of both 3'-hydroxydaidzin and 3'-hydroxygenistin.
關聯:	Molecules, v.21 n.12, 1723
显示于类别:	[生物科技系(所)] 期刊論文

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