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https://ir.cnu.edu.tw/handle/310902800/30913
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| 標題: | RNA polymerase B subunit gene mutations in biofilm-embedded methicillin-resistant Staphylococcus aureus following rifampin treatment |
| 作者: | Tang, Hung-Jen
Lai, Chih-Cheng
Hsueh, Po-Ren
Chen, Chi-Chung
Wu, Kuan-Ying
Lin, Yi-Chung
Zhang, Chun-Cheng
Weng, Tzu-Chieh
Chiu, Yu-Hsin
Toh, Han-Siong
Chiang, Shyh-Ren
Yu, Wen-Liang
Ko, Wen-Chien
Chuang, Yin-Ching |
| 貢獻者: | Chi Mei Med Ctr, Dept Med
Chia Nan Univ Pharm & Sci, Dept Hlth & Nutr
Chi Mei Med Ctr, Dept Intens Care Med
Natl Taiwan Univ Hosp, Dept Lab & Internal Med
Natl Taiwan Univ, Coll Med
Chi Mei Med Ctr, Dept Med Res
Natl Cheng Kung Univ, Med Coll & Hosp, Inst Biotechnol
Chi Mei Med Ctr, Dept Internal Med
Natl Cheng Kung Univ, Med Coll & Hosp, Dept Med |
| 關鍵字: | biofilm-embedded MRSA
mutations
rpoB gene |
| 日期: | 2016-06 |
| 上傳時間: | 2018-01-18 11:37:49 (UTC+8) |
| 出版者: | Elsevier Taiwan |
| 摘要: | Background/Purpose: This study was conducted to compare the mutation rates of different rpoB sites and rifampin minimum inhibitory concentration (MIC) changes prior to and after rifampin therapy for biofilm-embedded methicillin-resistant Staphylococcus aureus (MRSA) isolates. Methods: The screening of rifampin-resistant MRSA isolates, from the biofilm at Day 5 with or without exposure to the susceptible breakpoint concentration of rifampin recommended by the Clinical and Laboratory Standards Institute (1 mg/L), was conducted using agar plates containing rifampin. A partial fragment of RNA polymerase B subunit gene (rpoB), including clusters I and II, was amplified and sequenced. The rifampin MIC values and mutation frequencies at different sites of rpoB were measured and evaluated in rifampicin-resistant isolates. Results: Rifampin-resistant mutants could be selected from all of 39 randomly selected rifampin-susceptible MRSA isolates in the biofilm model. The spontaneous mutation frequency ranged from 1.00 x 10(-4) to 3.85 x 10(-7). Mutation at codon 481 was most commonly found at 35 (89.7%) of 39 MRSA isolates. Without rifampin induction, the MIC ranged between 0.125 mg/L and 1024 mg/L and mutation sites included cluster I 464, 466, 468, 471, 474, 477, 481, 484, 486 and cluster II 519, 527, 529 with the percentage of 471 (35.9%), 477 (33.3%), 481 (53.8%), and 484 (35.9%). Conversely, with the induction of rifampin, the MIC value ranged similar to 256-1024 mg/L. The mutation sites that were more concentrated included 468 (17.9%), 477 (30.8%), 481 (89.7%), 484 (17.9%), and 486 (33.3%). Conclusion: We documented high rifampin resistance induction activity when MRSA was engaged in biofilm with rifampin exposure. Monotherapy seems to be inadequate for MRSA in biofilm. There is an urgent need for developing effective combination therapies with less rifampin resistance-inducing activities for treating MRSA in biofilms. Copyright (C) 2015, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. |
| 關聯: | Journal of Microbiology Immunology and Infection, v.49 n.3, pp.394-401 |
| 顯示於類別: | [保健營養系(所) ] 期刊論文
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