本研究，對於高雄港口海洋沉積污染物，引進生物處理進行半批次有氧實驗替代化學處理。其目的係通過使用受污染土壤製備之細菌混合物，來研究分離菌去除沉積物中之化學需氧量與多環芳香烴之可能性。細菌混合物由三種特定可降解柴油之細菌組成，包括無色桿菌、念珠菌屬以及從柴油儲罐中分離用於柴油污染土壤處理之黃色多醣酵母。實驗採用四種不同實驗方式進行，有含及不含合成營養液與港口沉積物，以研究細菌混合物與沈積物中微生物之化學需氧量去除效率。在每次運行結束時，通過使用浸沒微濾中空纖維膜吸收處理過之溶液，以保留並濃縮反應器內之微生物，然後根據實驗設計，另用新之營養液替代。結果顯示，海域沉積物中在有氧條件下，分離菌降解了溶解性化學需氧量(20.15%)，去除率最高之苯并[b]丙二烯合茀(BbF)為86.19%。港口沉積物中之微生物降解了溶解性化學需氧量(14.24±3.5%)與幾乎所有之多環芳香烴，最高去除率為?(An)之92.05%。添加營養液之實驗除去了高分子多環芳香烴，分別除去氯(Chr)、苯并[b]丙二烯合茀(BbF)、苯并[k]丙二烯合茀(BkF)；分別為21.29、42.21與61.38%，溶解性化學需氧量之去除率為41.66±14.24%。經過處理之沉積物細胞毒性對正常細胞（選殖9號）與癌細胞(HepG2) 之細胞存活率影響，其中最高之正常細胞存活率為海水沉積物中微生物沉積物處理之71.73%，最高癌細胞存活率為經分離之細菌與營養液處理沉積物之93.16%。分離菌與土著菌之性能，其能夠將港口沉積物中之溶解性化學需氧量與多環芳香烴移除。未來，微生物群落應進行分析研究，瞭解有機污染沉積物降解之主要微生物。 In this study, aerobic batch experiments were conducted in order to introduce a biological treatment instead of chemical treatment for contaminated harbor sediment of Kaohsiung harbor. The aim of the study was to investigate the possibility of mixed culturebacteria to remove COD and PAHs in the sediment by using a bacteria mixture prepared by the polluted-contaminated soil. The bacteria mixture consists of three specific diesel-degradable bacteria including Achromobacter insolitus, Candida spp., and Xanthobactor polyaromatici yorans which are isolated from diesel storage tank for diesel contaminated soil treatment. The experiments were carried out with 3 different experiments with/without synthetic nutrient solution and harbor sediment to investigate the COD removal efficiency of bacteria mixture and the microbes existed in sediment. In the end of each runs, the treated solution was sucked by using submerged MF hollow fiber membrane to retain and enrich the microbes inside of the reactor and then replaced with a new nutrient solution depending the experiment design. The results showed that the mixed culturebacteria degraded SCOD (20.15%) and the highest PAHs removal was 86.19% of benzo[b]fluoranthene (BbF) in harbor sediment under aerobic condition. The microorganism in harbor sediment degraded SCOD (14.24?3.5%) and almost all PAHs, which the highest removal was 92.05 % of anthracene (An). The experiment with the nutrient solution addition removed high molecular PAHs, the removal of chrysene (Chr), benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF) was 21.29, 42.21, and 61.38%, respectively and SCOD removal was 41.66?14.24%. The effect of cytotoxic of treated sediment on cell viability of normal cells (Clone 9) and cancer cells (HepG2), which the highest normal cells viability was 71.73% of sediment treatment by microorganism in harbor sediment and the highest cancer cells viability was 93.16% of treated sediment with mixed culture bacteria and nutrient solution. The performances of mixed culture and indigenous bacteria were able to remove PAHs and SCOD from harbor sediment. In the future, microbial community should be studied on the analysis to understand the dominate microorganism for the degradation of organic-contaminated sediment.
