高雄港沉積物受到多環芳香烴(PAHs)和總有機碳之污染，嚴重影響了水中環境。本研究目的，係為調查我們從柴油儲罐分離之實驗室中用於洗滌柴油之表面活性劑而受污染之土壤，其土著菌和細菌混合物之生物利用度。此外，還對高雄港口經處理後之沉積物，在缺少氧及細胞活動力之情況下，其多環芳香烴之去除能力進行研究。在實驗批次中設置四種不同之實驗條件：(1) 含RO水之沉積物、(2) 含RO水之沉積物接種外源細菌、(3) 含營養液之外源細菌、(4) 含營養液之沉積物接種外源細菌；所有實驗均在缺氧反應中進行，以研究細菌對高雄港污染沉積物進行修復之生物利用度。在反應中，通過使用側流膜來代替營養液，用以將細菌濃縮。結果顯示，沉積物COD最高去除效率係以營養餵養細菌混合物約49％。土著菌可以生物降解二與三環多環芳烴、13-33％、高環多環芳烴、57%之丙二烯合茀與73％之芘。沒有營養液之外源細菌可以生物降解高環多環芳烴、53％之芘與69％之苯并[a]芘。營養液外源細菌可降解低環和高環多環芳烴，特別是高達50-100％之高環。細胞毒性分析發現，在沒有營養液餵養之外源性細菌條件下，觀察到正常肝細胞（選殖9號）之存活率最高。結果顯示，土著菌在低溫高環多環芳烴生物降解中有效，外源細菌對生物降解高環多環芳香烴有效。 Kaohsiung harbor sediment was significantly contaminated with polycyclic aromatic hydrocarbons (PAHs) and total organic carbons, which affected the aquatic environment. The aim of this study was to investigate the bioavailability of indigenous bacteria and bacteria mixture (Achromobacter insolitus, Candida spp. and Xanthobacter polyaromaticivorans) from our laboratory isolated from diesel storage tank, which was used for the treatment of diesel contaminated soil washing by a surfactant. In addition, PAHs removal from Kaohsiung harbor sediment under anoxic condition and the cell viability of treated harbor sediment were also investigated. The batch experiments were set up with 4 different conditions; (1)sediment with RO water, (2)sediment inoculated exogenous bacteria with RO water, (3)exogenous bacteria with nutrient solution, (4)sediment inoculated exogenous bacteria with nutrient solution, all experiment were conducted in an anoxic reactor to study the bioavailability of bacteria for remediation of contaminated sediment from Kaohsiung Harbor. The nutrient solution was replaced by using a side-stream membrane to concentrate the bacteria in the reactor. The results showed that the highest removal efficiency of COD of sediment was around 49% of bacteria mixture with nutrient feeding. The indigenous bacteria could biodegrade both LMW and HMW PAHs, 57% of Fluoranthene, 73% of pyrene and 100% of Benzo[b]fluoranthene, Benzo[k]fluoranthene and Benzo[a]pyrene. The exogenous bacteria without nutrient solution could transform HMW PAHs, 53% of chrysene and 69% of Benzo[a]pyrene. The exogenous bacteria with nutrient solution could degrade both LMW and HMW PAHs, especially HMW up to 50-100%. The highest viability of normal cell (clone9) was observed at the condition of exogenous bacteria without nutrient solution feeding. The results revealed that the indigenous bacteria was efficient in biodegrade both LMW and HMW PAHs with 62% total removal efficiency, the exogenous bacteria could biodegrade HMW PAHs, however it was not the most effective in biodegradation of PAHs in harbor sediment.
