| 摘要: | 中草藥植物近年來成為主要原料開發的方向，其萃取物是新一代化粧品配方中常用的添加物，本研究以一些中草藥之乙醇萃取物來評估與探討抗菌活性，透過實驗數據來驗證這些萃取物對於金黃色葡萄球菌、綠膿桿菌和大腸桿菌的抗菌效能。
抗菌活性經由紙錠擴散法試驗結果顯示，五倍子萃取物對於金黃色葡萄球菌抑制效果最佳，抑菌圈大小為14.7±0.5mm，丁香水萃取物對於大腸桿菌抑制效果最佳，抑菌圈範圍可到13.3±1.5mm。而在最小抑菌濃度試驗(MIC)與最小殺菌濃度試驗(MBC)之結果顯示，五倍子萃取物對於金黃色葡萄球菌有最佳的抑菌效果，MIC值與MBC值分別為0.02%及0.09%，其對於大腸桿菌也具有較佳的抑菌效果，MIC值與MBC值則分別為0.15%及0.52%;在丁香和紅球薑乙醇萃取物中，均對於綠膿桿菌擁有最佳的抗菌效果，MIC分別為0.25%與0.25%，而MBC值分別為0.26%和0.35％。結論是五倍子萃取物對於金黃色葡萄球菌及大腸桿菌的抑菌效果皆較好，丁香及紅球薑乙醇萃取物則對綠膿桿菌之抗菌效果較佳。因此，將五倍子、丁香和紅球薑之乙醇萃取物進行特定比例混合成複方型式抗菌劑，再經由D值線性迴歸法試驗評估，求得金黃色葡萄球菌、綠膿桿菌與大腸桿菌在自製精華液配方中求得D值的時間大小均小於4小時，在三組複方型式抗菌劑中對於金黃色葡萄球菌D值約在1.4至2.4小時，對於綠膿桿菌D值約1.5至2.3小時，對於大腸桿菌D值則約為1.9至3.3小時。
In recent years, Chinese herbal plants have become a main direction of development on cosmetic materials because the ethanol extracts should be the most common additives in new cosmetic formulas. The aim of this study is to assess the antibacterial activity from some Chinese herbal extracts. The experimental results would verify the antibacterial effect on Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli.
Antibacterial activity was determined with the disc-diffusion assay, the results showed that ethanol extracts of Rhus chinensis Mill exhibited the best inhibitory effect on S. aureus, and the diameter of inhibitory zone was 14.7?0.5mm. The aqueous extracts of Syzygium aromaticum showed better inhibitory zone with 13.3?1.5mm on E. coli. In the experiments of minimum inhibitory concentration and the minimum bactericidal concentration, the results showed the ethanol extracts of Rhus chinensis Mill for S. aureus had the best antibacterial effect, with a MIC value of 0.02% and MBC value of 0.09%, individually. Furthermore, Rhus chinensis Mill extract for E. coli also had the efficacy, the MIC and MBC value were 0.15% and 0.52%, respectively. However, the ethanol extracts from Syzygium aromaticum, Zingiber zerumbet and Rhus chinensis Mill were all displayed in good antibacterial effects against P.aeruginosa, with the MIC value of 0.25%, 0.25% and 0.5%; and with the MBC value of 0.26%, 0.35 and 0.54%, respectively. In conclusion, the experimental data demonstrated the ethanol extracts of Rhus chinensis Mill had better antibacterial effect on S. aureus and E. coli; the ethanol extracts of Syzygium aromaticum and Zingiber zerumbet showed the best antibacterial effect on P.aeruginosa. As well, the ethanol extracts of Syzygium aromaticum, Zingiber zerumbet and Rhus chinensis Mill were made into three antibacterial complexes adding to the essence product with special ratio and were evaluated the antibacterial efficacy by the D-value method. Finally, we acquired the D-value time all within 4 hours on S. aureus, P. aeruginosa and E. coli in the essence of the formula. The D-value time of three antibacterial complexes for S. aureus, P. aeruginosa and E. coli were from 1.4 to 2.4 hrs, from 1.5 to 2.3 hrs, and from 1.9 to 3.3 hrs, respectively. |
