台灣化粧品原料來源大都仰賴進口,本土原料相當缺乏,因此化粧品產業極需有本土創新原料的開發,本論文將針對海洋及溫泉等資源,進行原料開發。開發內容包含各類水源以及具有潛力之微生物,篩選策略以蛋白質分解能力作為依據,利用牛奶中的蛋白質做為初步的判斷,從溫泉中分離出的菌種和海泥中分離出的菌種,判斷其是否具有分解蛋白質之酵素。再搭配保濕性測試並於其中選出效力最佳的菌種做鑑定。保濕測試之中,在不同濃度的海水中,可以提供20 % 的保濕增加率,角質貼片試驗中,與保濕測試相對照,當保濕增加率越高,被撕下之角質層相對越少。牛奶澄清試驗,在不同海水之中,牛奶濁度在2小時後,下降91 % 。總結:本論文開發各類可應用於化粧品之資源,期望能提供國內原料商及相關產業對於創新化粧品原料開發之新觀念。 Taiwan cosmetics raw materials mostly rely on imports, the local raw materials is quite lacking, so the cosmetics industry is extremely necessary to have the development of local innovative raw materials, this paper will be for the marine and hot springs and other resources, raw materials development.The development content includes all kinds of water sources and potential microbes. The screening strategy is based on the ability of protein decomposition, and the protein in milk is used as the preliminary judgment. The strains isolated from the hot spring and the bacteria isolated from the sea mud, To determine whether it has an enzyme that breaks down the protein. And then with the moisture test and selected in which the effectiveness of the best bacteria to do identification.From the hot springs and seabed mud isolated with a moisturizing bacteria species, the use of milk clarification test, screening of special strains, showing the value of cosmetics applications.Moisturizing test, in different concentrations of seawater can provide 20% moisture increase rate. In contrast to the moisturizing test, when the moisturizing increase rate is higher, the tearing of the stratum corneum is relatively less. Milk clarification test, in different seawater down 91%, but after 90 ℃ heating 1 hour, the rate of decline in turbidity.Summary: This paper develops all kinds of resources applicable to cosmetics, and hopes to provide new ideas for the development of innovative raw materials for raw materials and related industries.