添加金屬對菸鹼酸衍生物對電化學的反應

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Title:	添加金屬對菸鹼酸衍生物對電化學的反應
Authors:	林詠樺
Contributors:	嘉南藥理大學醫藥化學系王來好
Date:	2013
Issue Date:	2017-12-13 11:28:00 (UTC+8)
Abstract:	添加金屬對菸鹼酸衍生物{菸鹼酸 ( Nicotinic acid )、菸酸醯胺 ( Nicotinamide )、菸酸氧肟酸 ( Nicotinic acid hydroxamate )等}對電化學的反應。利用電化學儀器、高效液相層析儀串連電化學偵測器 ( High performance liquid chromatography – Electrochemical，HPLC-ECD )並串聯光二極體陣列偵檢器 ( Photo-Diode Array Detector )。探討最佳的層析條件，如層析條件：改變緩衝溶液比例和其pH 值、濃度，看添加入的金屬是否會和菸鹼酸的衍生物產生化學反應。而添加入金屬與菸鹼酸衍生物於電化學就無電流值 (μA )上的變化，進而改變以工作電極為含硫醇藥品薄膜修飾銀電極提升其感度與文獻上工作電極為金電極做比較。進一步利用高效液相層析儀串連電化學偵測器 ( High performance liquid chromatography – Electrochemical- Ultraviolet，HPLC-ECD-UV ) 找出適合的電位並加以論證。實驗探討研究結果顯示於層析條件為移動相：甲醇 ( Methanol ) : H2O [ Phosphate buffer ( pH =4 )] = 20 : 80 為最適化條件。以直流電模是固定於一電位下，線性範圍濃度從0.05 ~ 8 μg/mL。發現使用HPLC-ECD 比HPLC- UV 感度明顯增加。 The method was based on the instrument of electrochemical, high performance liquid chromatography – electrochemical(HPLC-ECD) and connect photo-diode array detector to determine the metal be added to Nicotinic acid derivative {Nicotinic acid, Nicotinamide, Nicotinic acid hydroxamate etc}. Discussion optimal chromatographic conditions. The results showed no change in the current value after the reaction of metal and nicotinic acid derivatives for electrochemical. So changing the working electrode modified with thiol-containing drug film silver electrode to enhance their sensitivity and comparing the literature of the gold electrode. The experimental conditions for the chromatographic mobile phase: Methanol: H2O [Phosphate buffer (pH = 4)] = 20: 80 as the optimal conditions.For direct current mode, with the current at a constant potential, and measurements with suitable experimental parameters, we found a linear concentration range from 0.05 to 8.0 μg mL?1 .Findings using HPLC-ECD and HPLC with an ultraviolet detector were comparable.
Relation:	計畫編號：102-2815-C-041-001-M 計畫年度：102；執行起迄：2013-07-01~2014-02-28
Appears in Collections:	[Dept. of Food & Drug Industry and Inspective Technology] Bachelor's Monographs

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