Piceatannol stimulates osteroblast differentiation that may be mediated by increased bone morphogeneric protein-2 production

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標題:	Piceatannol stimulates osteroblast differentiation that may be mediated by increased bone morphogeneric protein-2 production Piceatannol 可能透過增加BMP-2 的產物的方式刺激調節成骨細胞的分化做愈
作者:	I-Chun Teng(鄧一君) Ya-Ling Hsu(許雅玲) Po-Lin Kuo(郭柏麟) Jiunn-Kae Chang(張竣凱)
貢獻者:	Cell Biology Laboratory, Department of Biotechnology, Chia-Nan University of Pharmacy and Science
日期:	2008-07
上傳時間:	2017-12-04 16:00:47 (UTC+8)
摘要:	Background and purpose: We examined the effect of piceatannol on the proliferation and differentiation of osteoblast cells. Piceatannol stimulates osteoblast differentiation that may be mediated by increased bone morphogenetic protein-2production.Methods:Inhibition of cell proliferation by piceatannol was measured by XTT assay; By means of alkaline phosphatase activity ,osteocalcin enzyme-linked immunosorbent assay (ELISA) and we have shown that piceatannol exhibits a signifgicant induction of differentiation; The degree of mineralization was determined using Alizarin Red staining ; The type I procollagen assay, which measures the propeptide portion of the molecule and reflects the synthesis of the mature form of the protein; The role of BMP-2 in piceatannol-mediated cell differentiation was determined using assaying the levels of BMP-2; Expression levels of BMP-2, alkaline phosphatase and osteocaclin mRNA were examined using RT-PCR. Results: Piceatannol stimulate osteoblast differentiation at various stages (from maturation to terminally differentiated osteoblasts). Induction of differentiation by piceatannol was associated with increased bone morphogenetic protein-2 (BMP-2) production. Addition of purified BMP-2 protein did not increase the upregulation of alkaline phosphatase activity and osteocalcin secretion by piceatannol, whereas the BMP-2 antagonist noggin blocked piceatannol and BMP-2-mediated alkaline phosphatase activity, and osteocalcin secretion enhancement, indication that BMP-2 production is required in piceatannol-mediated osteoblast maturation and differentiation. Conclusions: Piceatannol increased BMP-2 synthesis, and this effect may contribute to its action on the induction of osteoblasts maturation and differentiation, followed by an increase of bone mass. Decreases in new bone formation, followed by estrogen deficiency or various pathologic factors, may contribute to the mechanisms involved in Postmenopausal osteoporosis.
關聯:	第五屆海峽化學、生物及材料研討會，起迄日：2008/07/21-2008/07/22，地點：嘉南藥理科技大學
Appears in Collections:	[生物科技系(所)] 會議論文 [藥理學院] 2008第五屆海峽化學、生物及材料研討會

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