本研究設計新電化學技術，製配5種硫醇(thiol)化合物薄膜 (2, 4-thiazolidinedione, 2-mercapto-5-thiazoline, 2-mercaptothiazoline, L-cysteine, thioglycolic acid)，修飾金電極當工作電極。裝入流動式電解槽 (flow cell)中，當作檢測器並連線高效率液相層析儀，測定甲硒胺酸 (L-elenomethionine)和甲基硒代半胱胺酸 (Se-methylseleno-L-cysteine)之含量。二種有機硒的電化學氧化效果在硫醇(thiol)修飾金電極測定之靈敏度皆比
未修飾金電極為高。利用DC模式，合適的實驗參數測定，線性濃度從10至1600 ng mL1。二種有機硒的偵測極限低於10 ng mL1。此方法可以適用於定量測定市售含有硒補充產品之甲硒胺酸和甲基硒代半胱胺酸。比較高效率液相層析法連線伏安檢測器和紫外線檢測之結果是一致。 A flow-electrolytical cell containing a thio-compounds (2,
4-thiazolidinedione, 2-mercapto-5-thiazoline, 2-
mercaptothiazoline, L-cysteine, thioglycolic acid) modified
gold electrodes has been designed and characterized for use
in a voltammatric detector for detecting L-selenomethionine
and Se-methylseleno-L-cysteine using high-performance
liquid chromatography. Both L-selenomethionine and Semethylseleno-
L-cysteine are more
efficiently electrochemical oxidized on a thio/ Au than a
bare gold electrode. For the DC mode, and measurements with
suitable experimental parameters, a linear concentration
from 10 to 1600 ng mL?1 was found. The limit of
quantification for L-selenomethionine and Se-methylseleno-
L-cysteine were below 10 ng mL?1. The method can be applied
to the quantitatively determining L-selenomethionine and
Se-methylseleno-L-cysteine in commercial containing
selenium supplement products. Findings using highperformance
liquid chromatography with a flow-through
voltammetric detector and ultraviolet detector are
comparable.
